Ander Stephanie E, Davenport Bennett J, Evans Alyssa B, Morrison Thomas E
Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, USA.
Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA.
Methods Mol Biol. 2025;2893:285-298. doi: 10.1007/978-1-0716-4338-9_21.
During viral infection, phagocytic cells participate in numerous immunological processes. A common approach to elucidate the specific contributions of phagocytic cells is through direct comparison of viral pathogenesis of phagocyte-depleted and phagocyte-intact animals. Clodronate liposomes are a versatile and simple means of depleting phagocytes in any animal model. Selection of clodronate liposome route of delivery can yield systemic or local phagocyte depletion as needed. Here we describe the application of clodronate liposome-mediated depletion of blood-contacting phagocytes to investigate their interactions with La Crosse virus (or other bunyaviruses) in a reductionist model of viremia.
在病毒感染期间，吞噬细胞参与众多免疫过程。阐明吞噬细胞具体作用的一种常用方法是直接比较吞噬细胞缺失和吞噬细胞完整的动物的病毒发病机制。氯膦酸脂质体是在任何动物模型中消耗吞噬细胞的一种通用且简便的方法。根据需要选择氯膦酸脂质体的给药途径可实现全身或局部吞噬细胞的消耗。在这里，我们描述了应用氯膦酸脂质体介导的血液接触吞噬细胞的消耗，以在病毒血症的简化模型中研究它们与拉克罗斯病毒（或其他布尼亚病毒）的相互作用。
