腺相关病毒介导的微小RNA在大鼠心力衰竭模型中的递送方案。
Protocol for adeno-associated virus-mediated miRNA delivery in a rat heart failure model.
作者信息
Mushtaq Iqra, Kao Yu-Hsun, Chen Yi-Jen
机构信息
International Ph.D. Program in Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.
International Ph.D. Program in Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan; Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan; Department of Medical Education and Research, Wan Fang Hospital, Taipei Medical University, Taipei 11031, Taiwan.
出版信息
STAR Protoc. 2024 Dec 20;5(4):103498. doi: 10.1016/j.xpro.2024.103498. Epub 2024 Dec 12.
Delivering microRNA (miRNA) to treat cardiac disease is a significant challenge, and selecting an efficient delivery method to target the affected organ is critical for therapeutic success. Here, we present a protocol for delivering miRNA to the heart of rats with heart failure using adeno-associated virus (AAV9) coupled with a hydrodynamic transfusion strategy. We describe steps for inducing heart failure, echocardiography, and AAV9 application. Further, we provide a comprehensive procedure to detect miRNA expression in cardiac tissues using stem-loop RT-qPCR. For complete details on the use and execution of this protocol, please refer to Mushtaq et al..
递送微小RNA（miRNA）来治疗心脏病是一项重大挑战，选择一种有效的递送方法以靶向受影响器官对于治疗成功至关重要。在此，我们展示了一种使用腺相关病毒（AAV9）并结合流体动力学输血策略将miRNA递送至心力衰竭大鼠心脏的方案。我们描述了诱导心力衰竭、超声心动图检查和应用AAV9的步骤。此外，我们提供了一种使用茎环逆转录定量聚合酶链反应（stem-loop RT-qPCR）检测心脏组织中miRNA表达 的全面程序。有关此方案的使用和执行的完整详细信息，请参考穆斯塔克等人的研究。
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