Wang Hui, Bi Duohang, Yu Bowen, Chen Qiang, Du Shuo, Xie Ge, Zhu Jintao, Zhang Lianbin
Key Lab of Material Chemistry for Energy Conversion and Storage of Ministry of Education, Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan 430074, China.
Key Lab of Material Chemistry for Energy Conversion and Storage of Ministry of Education, Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan 430074, China.
J Colloid Interface Sci. 2025 Mar 15;682:1185-1194. doi: 10.1016/j.jcis.2024.12.018. Epub 2024 Dec 6.
Photodynamic therapy (PDT) offers potential for combating bacterial infections through the generation of reactive oxygen species (ROS). However, the antibacterial efficiency of PDT is largely impeded by the limited photon absorption of photosensitizers and the short diffusion length and lifespan of ROS. Herein, we present a light-harvesting platform based on l-arginine-modified photonic hydrogels loaded with new indocyanine green (PG@Arg/IR820) for synergizing the slow photon effect with NO gas therapy to enhance PDT antibacterial efficiency. Upon near-infrared (NIR) light irradiation, PG@Arg/IR820 can maximize the utilization of photons via the slow photon effect to generate sufficient ROS, which not only acts as the primary bactericidal agent in PDT but also triggers l-arginine to generate NO. NO exhibits a long diffusion distance and lifespan and can freely diffuse to inhibit distant bacterial growth, demonstrating a vital complementary advantage in bacterial inactivation by ROS. The synergistic effect of the slow photon effect combined with NO gas therapy allows PG@Arg/IR820 to intensify bacterial destruction and enhance PDT antibacterial efficiency. This antibacterial system sheds light on an advisable design principle for efficient antibacterial activities in photodynamic inactivation.
光动力疗法（PDT）通过产生活性氧（ROS）为对抗细菌感染提供了潜力。然而，PDT的抗菌效率在很大程度上受到光敏剂有限的光子吸收以及ROS短扩散长度和寿命的阻碍。在此，我们提出了一种基于负载新型吲哚菁绿的l-精氨酸修饰光子水凝胶（PG@Arg/IR820）的光捕获平台，用于将慢光子效应与NO气体疗法协同作用以提高PDT抗菌效率。在近红外（NIR）光照射下，PG@Arg/IR820可通过慢光子效应最大化光子利用以产生足够的ROS，ROS不仅作为PDT中的主要杀菌剂，还能触发l-精氨酸产生NO。NO具有长扩散距离和寿命，可自由扩散以抑制远处细菌生长，在ROS使细菌失活过程中显示出重要的互补优势。慢光子效应与NO气体疗法的协同作用使PG@Arg/IR820能够增强细菌破坏并提高PDT抗菌效率。该抗菌系统为光动力失活中高效抗菌活性提供了一种明智的设计原则。
