Wang Junmei, Li Jianling, Han Hua, Wang Changhua, Shi Taiying, Yang Xueyun
Department of Ultrasound 2, Xinxiang Central Hospital (The Fourth Clinical College of Xinxiang Medical University), Xinxiang, 453000, China.
Department of Ultrasound 2, Xinxiang Central Hospital (The Fourth Clinical College of Xinxiang Medical University), Xinxiang, 453000, China.
Mol Cell Probes. 2025 Feb;79:101999. doi: 10.1016/j.mcp.2024.101999. Epub 2024 Dec 19.
Due to the complex pathogenesis of endometriosis, its early screening and development prediction are still challenging problems in the clinic.
This study evaluated the significance of miR-375-3p in endometriosis onset, progression, and recurrence, aiming to identify a novel biomarker for disease diagnosis and prognosis.
The study enrolled 100 patients with endometriosis and 80 healthy females. The serum miR-375-3p levels were compared between the two groups, and its diagnostic significance and predictive value were assessed by ROC and Cox regression analyses. The effect of miR-375-3p on endometriosis cell growth and motility was evaluated by CCK8 and Transwell assays.
Endometriosis patients showed a lower serum miR-375-3p level relative to healthy females, and more severe the disease condition, lower the miR-375-3p in endometrial tissues is. Reducing serum miR-375-3p could discriminate endometriosis patients sensitively and specifically. Additionally, miR-375-3p was identified as a predictor for the recurrence of endometriosis together with stage, lesion size, and the levels of related hormones. In endometriosis cells, miR-375-3p was demonstrated to target NOX4 and negatively regulated its expression. Overexpressing miR-375-3p significantly suppressed cell proliferation, migration, and invasion, which was reversed by NOX4.
Decreasing miR-375-3p served as a biomarker for endometriosis onset, development, and recurrence. miR-375-3p regulated endometriosis cell growth and motility via negatively modulating NOX4.
由于子宫内膜异位症发病机制复杂，其早期筛查和病情发展预测仍是临床上面临的难题。
本研究评估miR-375-3p在子宫内膜异位症发病、进展及复发中的意义，旨在寻找一种新的疾病诊断和预后生物标志物。
本研究纳入100例子宫内膜异位症患者和80例健康女性。比较两组血清miR-375-3p水平，并通过ROC曲线和Cox回归分析评估其诊断意义和预测价值。采用CCK8和Transwell实验评估miR-375-3p对子宫内膜异位症细胞生长和迁移能力的影响。
与健康女性相比，子宫内膜异位症患者血清miR-375-3p水平较低，且病情越严重，子宫内膜组织中miR-375-3p水平越低。降低血清miR-375-3p可敏感且特异地鉴别子宫内膜异位症患者。此外，miR-375-3p与分期、病灶大小及相关激素水平共同被确定为子宫内膜异位症复发的预测指标。在子宫内膜异位症细胞中，miR-375-3p被证明靶向NOX4并负向调节其表达。过表达miR-375-3p可显著抑制细胞增殖、迁移和侵袭，而NOX4可逆转这种作用。
miR-375-3p水平降低可作为子宫内膜异位症发病、发展及复发的生物标志物。miR-375-3p通过负向调节NOX4来调控子宫内膜异位症细胞的生长和迁移能力。
