以烷基溶血磷脂为底物的O-烷基裂解酶新检测方法。
New assay for the O-alkyl cleavage enzyme with alkyl-lysophospholipids as substrates.
作者信息
Unger C, Eibl H, von Heyden H W, Nagel G A
出版信息
Cancer Res. 1985 Feb;45(2):616-8.
PMID:3967236
Abstract
The O-alkyl cleavage enzyme is important for the metabolism of cytotoxic alkyl-lysophospholipids. We have developed a simple new method for the determination of the enzyme activity which is based on the formation of water-soluble phosphate during the enzyme reaction. This is the first assay which avoids the use of radiolabeled substrates.
摘要
O-烷基裂解酶对细胞毒性烷基溶血磷脂的代谢很重要。我们开发了一种简单的新方法来测定该酶的活性，该方法基于酶反应过程中水溶性磷酸盐的形成。这是第一种避免使用放射性标记底物的检测方法。
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