Sladek N E, Low J E, Landkamer G J
Cancer Res. 1985 Feb;45(2):625-9.
The sensitivity of cultured L1210 and P388 cells, sensitive (L1210/0, P388/0) and resistant (L1210/CPA, P388/CPA) to cyclophosphamide in vivo, to five oxazaphosphorine and eight nonoxazaphosphorine cross-linking agents was determined. Each of the resistant sublines was cross-resistant to all of the oxazaphosphorines tested. The P388/CPA cell line was also cross-resistant to all of the nonoxazaphosphorines but, in most cases, not nearly to the same extent. The L1210/CPA cell line was collaterally sensitive to all but one of the nonoxazaphosphorines, in which case it was equisensitive. Changes in sensitivity could not be accounted for by changes in intracellular pH values, or by changes in intracellular inorganic phosphate or acid-soluble organic phosphate concentrations. Inasmuch as the L1210/CPA cell line was specifically resistant to the oxazaphosphorines, identification of the phenotypic basis for this resistance should serve to identify a potentially important determinant with regard to the basis for the oncotoxic specificity of this group of agents.
测定了体内对环磷酰胺敏感（L1210/0、P388/0）和耐药（L1210/CPA、P388/CPA）的培养L1210和P388细胞对五种氧氮磷杂环类药物和八种非氧氮磷杂环类交联剂的敏感性。每个耐药亚系对所有测试的氧氮磷杂环类药物均呈交叉耐药。P388/CPA细胞系对所有非氧氮磷杂环类药物也呈交叉耐药，但在大多数情况下，耐药程度远不及对氧氮磷杂环类药物。L1210/CPA细胞系对除一种非氧氮磷杂环类药物外的所有药物呈侧链敏感，对该种药物则为同等敏感。敏感性的变化不能用细胞内pH值的变化、细胞内无机磷酸盐或酸溶性有机磷酸盐浓度的变化来解释。鉴于L1210/CPA细胞系对氧氮磷杂环类药物具有特异性耐药性，确定这种耐药性的表型基础应有助于确定这组药物致癌毒性特异性基础的一个潜在重要决定因素。
