Novick P, Botstein D
Cell. 1985 Feb;40(2):405-16. doi: 10.1016/0092-8674(85)90154-0.
The consequences of two different mutations in the single essential actin structural gene of yeast (Saccharomyces cerevisiae) were studied. Both conditional-lethal actin mutants exhibit six phenotypes at the restrictive temperature: disruption of the asymmetric staining pattern of actin assembly; delocalized deposition of chitin on the cell surface; partial inhibition of secretion of the periplasmic protein, invertase; an intracellular accumulation of secretory vesicles; death of cells in the budded portion of the cell cycle upon prolonged incubation at the restrictive condition; and osmotic sensitivity. These results implicate actin in the organization and polarized growth of the yeast cell surface.
对酵母（酿酒酵母）单个必需肌动蛋白结构基因中两种不同突变的后果进行了研究。两种条件致死型肌动蛋白突变体在限制温度下均表现出六种表型：肌动蛋白组装的不对称染色模式破坏；几丁质在细胞表面的异位沉积；周质蛋白转化酶分泌的部分抑制；分泌小泡的细胞内积累；在限制条件下长时间孵育后，细胞周期出芽部分的细胞死亡；以及渗透敏感性。这些结果表明肌动蛋白参与酵母细胞表面的组织和极性生长。
