van Furth R, van Schadewijk-Nieuwstad M, Elzenga-Claasen I, Cornelisse C, Nibbering P
Cell Immunol. 1985 Feb;90(2):339-57. doi: 10.1016/0008-8749(85)90199-6.
The aim of the present study was to obtain objective data on the morphology and quantitative information about other characteristics of murine macrophage-like cell lines J774.1, PU5-1.8, WEHI-3, and P388-D1, and to compare the findings with those in resident and exudate macrophages collected directly from mice. Fetal fibroblasts were included to serve as controls. Evaluation of the morphological data showed that the cell lines J774.1 and WEHI-3 are almost identical in most respects, that the cells of P388-D1 differ widely from both of the former lines, and that the morphometric parameters of cell line PU5-1.8 occupy an intermediate position. The cells of the P388-D1 line show the most similarity to resident and exudate macrophages, and cell lines J774.1 and WEHI-3 the least. Fetal fibroblasts had divergent values for all morphometric parameters. Good correspondence was found when the quantitative data obtained by morphometric analysis of the cells in question were compared with the morphological pictures. No gross differences as to cytochemical characteristics were found between the cells of the four cell lines, except for 5'-nucleotidase activity. The occurrence of IgG receptors and the ingestion of EIgG were also similar, but the percentage of cells with C3b receptors was much lower in two of the cell lines (WEHI-3 and P388-D1) and the level of EIgMC ingestion was very much higher in one (J774.1) compared with both the other cell lines and the resident and exudate macrophages. The ingestion of opsonized bacteria and latex varied widely within and between the cell lines. Quantitative data on the binding of monoclonal antibodies by the cells of the macrophage cell lines and the resident and exudate macrophages showed a wide variation. The doubling time of the cell lines is on average 1 day; distinct differences were found between these lines with respect to the lag-time of proliferation after replating. Cluster analysis and statistical analysis of morphological and other characteristics gave insight into the degree of resemblance between the cells of the four cell lines on the one hand and the resident and exudate macrophages on the other.
本研究的目的是获取关于小鼠巨噬细胞样细胞系J774.1、PU5-1.8、WEHI-3和P388-D1的形态学客观数据以及其他特征的定量信息，并将结果与直接从小鼠收集的驻留巨噬细胞和渗出巨噬细胞的结果进行比较。将胎儿成纤维细胞作为对照。对形态学数据的评估表明，细胞系J774.1和WEHI-3在大多数方面几乎相同，P388-D1细胞与前两个细胞系差异很大，细胞系PU5-1.8的形态测量参数处于中间位置。P388-D1细胞系的细胞与驻留巨噬细胞和渗出巨噬细胞最相似，而细胞系J774.1和WEHI-3最不相似。胎儿成纤维细胞的所有形态测量参数值都不同。当将通过对相关细胞进行形态测量分析获得的定量数据与形态学图片进行比较时，发现了良好的对应关系。除了5'-核苷酸酶活性外，在四个细胞系的细胞之间未发现细胞化学特征方面的明显差异。IgG受体的出现和EIgG的摄取也相似，但在两个细胞系（WEHI-3和P388-D1）中具有C3b受体的细胞百分比要低得多，而在一个细胞系（J774.1）中EIgMC摄取水平比其他细胞系以及驻留巨噬细胞和渗出巨噬细胞都高得多。细胞系对调理细菌和乳胶的摄取在细胞系内部和之间差异很大。巨噬细胞系细胞以及驻留巨噬细胞和渗出巨噬细胞与单克隆抗体结合的定量数据显示出很大差异。细胞系的平均倍增时间为1天；在重新接种后，这些细胞系在增殖的延迟时间方面存在明显差异。对形态学和其他特征的聚类分析和统计分析深入了解了四个细胞系的细胞与驻留巨噬细胞和渗出巨噬细胞之间的相似程度。
