Norouzinasab Fatemeh, Salimian Niloufar, Mokhtari Khatere, Akbari Mohammadarian, Maghsoudloo Mazaher, Entezari Maliheh, Taheriazam Afshin, Farahani Najma, Hashemi Mehrdad
Department of Genetics, Faculty of Advanced Science and Technology, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran; Farhikhtegan Medical Convergence Sciences Research Center, Farhikhtegan Hospital, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran.
Farhikhtegan Medical Convergence Sciences Research Center, Farhikhtegan Hospital, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran; Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Pathol Res Pract. 2025 Feb;266:155761. doi: 10.1016/j.prp.2024.155761. Epub 2024 Dec 9.
Colorectal cancer (CRC) is a prevalent malignancy worldwide, driven by complex molecular mechanisms. This study aims to elucidate the role of lncRNAs within TGF-β pathway, a crucial signaling pathway in CRC progression, focusing specifically on their interaction with the SPP1 gene. We employed a multi-faceted approach, starting with comprehensive in silico analyses to identify candidate lncRNAs potentially involved in TGF-β pathway regulation. These candidates were further validated through experimental RT-qPCR assays, comparing lncRNA expression profiles in CRC tissues to adjacent normal samples. Our findings revealed novel lncRNA candidates with significant associations with SPP1 in CRC, highlighting their potential regulatory roles in the TGF-β pathway. This integrative study underscores the importance of combining computational predictions with laboratory experimentation to uncover complex regulatory networks in cancer, providing insights into new therapeutic targets and diagnostic biomarkers for CRC.
结直肠癌（CRC）是一种在全球范围内普遍存在的恶性肿瘤，由复杂的分子机制驱动。本研究旨在阐明lncRNAs在TGF-β通路中的作用，TGF-β通路是CRC进展中的关键信号通路，特别关注它们与SPP1基因的相互作用。我们采用了多方面的方法，首先进行全面的计算机分析，以识别可能参与TGF-β通路调控的候选lncRNAs。通过实验性RT-qPCR分析进一步验证这些候选物，将CRC组织中的lncRNA表达谱与相邻正常样本进行比较。我们的研究结果揭示了在CRC中与SPP1有显著关联的新型lncRNA候选物，突出了它们在TGF-β通路中的潜在调控作用。这项综合性研究强调了将计算预测与实验室实验相结合以揭示癌症复杂调控网络的重要性，为CRC提供了新的治疗靶点和诊断生物标志物的见解。
