Dong Wanyuan, Wang Rongrong, Li Yifeng
Downstream Process Development (DSPD), WuXi Biologics, 31 Yiwei Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Downstream Process Development (DSPD), WuXi Biologics, 31 Yiwei Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Protein Expr Purif. 2025 Mar;227:106646. doi: 10.1016/j.pep.2024.106646. Epub 2024 Dec 12.
MabSelect VH3 is a new Protein A resin recently launched by Cytiva. According to the manufacturer, the Protein A ligand of MabSelect VH3 has been engineered to disrupt and reinforce its Fc and VH3 binding capabilities, respectively. Thus, different from regular Protein A resins, this new Protein A resin has affinity for VH3 domain only. The vendor has suggested that MabSelect VH3, owing to its unique selectivity, can separate byproducts that are different from the product in the number of VH3 domain. In the current work, with two concrete cases, we demonstrated that MabSelect VH3 indeed allows effective separation of species containing different numbers of VH3 domain. In addition, we showed that, in comparison to regular Protein A resins, MabSelect VH3 also exhibits improved aggregate separation potential. Thus, for cases where product and byproduct differ in the number of VH3 domain and/or culture harvest contains high percentage of aggregates, MabSelect VH3 is a better alternative than regular Protein A for product capture as it allows simultaneous removal of byproducts and aggregates.
MabSelect VH3是 Cytiva 最近推出的一种新型蛋白A树脂。据制造商称，MabSelect VH3的蛋白A配体经过改造，分别破坏并增强了其与Fc和VH3的结合能力。因此，与常规蛋白A树脂不同，这种新型蛋白A树脂仅对VH3结构域具有亲和力。供应商表示，由于MabSelect VH3具有独特的选择性，它可以分离出VH3结构域数量与产品不同的副产物。在当前的工作中，通过两个具体案例，我们证明了MabSelect VH3确实能够有效分离含有不同数量VH3结构域的物种。此外，我们还表明，与常规蛋白A树脂相比，MabSelect VH3在聚集物分离方面也具有更好的潜力。因此，对于产品和副产物在VH3结构域数量上存在差异以及/或者培养收获物中含有高比例聚集物的情况，MabSelect VH3在产品捕获方面是比常规蛋白A更好的选择，因为它能够同时去除副产物和聚集物。
