Chen Siqi, Zhang Wenxin, Zhang Qi, Li Bin, Zhang Mingzhe, Qin Jianchun, Shi Wuliang, Jia Chengguo
College of Plant Science, Jilin University, Changchun, 130062, China.
Plant Cell Rep. 2024 Dec 14;44(1):5. doi: 10.1007/s00299-024-03400-x.
SlNAC12 enhances salt stress tolerance of transgenic tomato by regulating ion homeostasis, antioxidant activity and flavonoids biosynthesis Soil salinization is a major environmental factor that adversely affects plant growth and development. NAC (NAM, ATAF1/2, and CUC2) is a large family of plant-specific transcription factors that play crucial roles in stress response. Here, we investigated the role of a novel NAC transcription factor, SlNAC12, in conferring salt stress tolerance in tomato (Solanum lycopersicum). Subcellular localization and yeast assays studies revealed that SlNAC12 is localized in the nucleus with weak transcriptional activity. SlNAC12 transcript was induced by salt stress in the leaves and roots of tomato seedlings. Overexpression of SlNAC12 in tomato led to significantly reduced plant height and root length. Transgenic tomato lines overexpressing of SlNAC12 (OE#1 and OE#3) exhibited enhanced tolerance to salinity, as evidenced by reduced the inhibitory effect of growth parameters under salt stress compared to wild type (WT). Overexpression of SlNAC12 in tomato affected Na and K homeostasis, leading to reduced Na/K ratio, enhanced activity of antioxidant enzymes and decreased reactive oxygen species (ROS) accumulation under salt stress. Furthermore, the transcript levels of several genes involved in flavonoids metabolism and the levels of flavonoids accumulation were increased in SlNAC12-overexpressing tomato lines. Collectively, this study suggests that SlNAC12 transcription factor enhances salt stress tolerance in tomato is correlated with ion homeostasis, antioxidant enzyme systems, and flavonoids accumulation.
SlNAC12通过调节离子稳态、抗氧化活性和类黄酮生物合成增强转基因番茄的耐盐性 土壤盐渍化是对植物生长发育产生不利影响的主要环境因素。NAC（NAM、ATAF1/2和CUC2）是一大类植物特异性转录因子，在应激反应中起关键作用。在此，我们研究了一种新型NAC转录因子SlNAC12在赋予番茄（Solanum lycopersicum）耐盐性方面的作用。亚细胞定位和酵母试验研究表明，SlNAC12定位于细胞核，转录活性较弱。番茄幼苗叶片和根中的SlNAC12转录本受盐胁迫诱导。番茄中SlNAC12的过表达导致株高和根长显著降低。与野生型（WT）相比，过表达SlNAC12的转基因番茄株系（OE#1和OE#3）对盐胁迫的耐受性增强，盐胁迫下生长参数的抑制作用降低证明了这一点。番茄中SlNAC12的过表达影响了钠和钾的稳态，导致盐胁迫下钠/钾比降低、抗氧化酶活性增强和活性氧（ROS）积累减少。此外，过表达SlNAC12的番茄株系中几个参与类黄酮代谢的基因的转录水平和类黄酮积累水平均有所增加。总的来说，这项研究表明，SlNAC12转录因子增强番茄耐盐性与离子稳态、抗氧化酶系统和类黄酮积累有关。
