Murali Athira, Parameswaran Ramesh
Department of Medical Devices Engineering, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
J Biomater Sci Polym Ed. 2025 Jun;36(8):987-1012. doi: 10.1080/09205063.2024.2436691. Epub 2024 Dec 15.
The implant surface chemistry and topography are primary factors regulating the success and survival of bone scaffold. Surface modification is a promising alternative to enhance the biocompatibility and tissue response to augment the osteogenic functionalities of polyesters like PLA. The study employed the synergistic effect of alkaline hydrolysis and polydopamine (PDA) functionalization to enhance the cell-material interactions on 3D printed polylactic acid (PLA) scaffold. Comprehensive characterization of the modified PLA highlights the improvements in the physical, chemical and cell-material interactions upon two-step surface modification. The X-ray photoelectron spectroscopy (XPS) analysis substantiated enhanced PDA deposition with ∼8.2% increase in surface N composition after surface etching due to homogeneous PDA deposition compared to the non-etched counterpart. The changes in surface chemistry and morphology upon dual surface modification complemented the human osteoblast (HOS) attachment and proliferation, with distinct cell morphology and spreading on PDA coated etched PLA (Et-PLAPDA) scaffolds. Moreover, substantial improvement in osteogenic differentiation of UMR-106 cells on etched PLA (Et-PLA) and Et-PLAPDA highlights the suitability of alkali etching-mediated PDA deposition to improve mineralization on PLA. Overall, the present work opens insights to modify scaffold surface composition, topography, hydrophilicity and roughness to regulate local cell adhesion to improve the osteogenic potential of PLA.
植入物表面化学性质和形貌是调节骨支架成功与存活的主要因素。表面改性是一种很有前景的方法，可增强生物相容性和组织反应，以增强聚乳酸（PLA）等聚酯的成骨功能。该研究利用碱水解和聚多巴胺（PDA）功能化的协同效应，来增强三维打印聚乳酸（PLA）支架上的细胞与材料的相互作用。对改性PLA的全面表征突出了两步表面改性后在物理、化学和细胞与材料相互作用方面的改善。X射线光电子能谱（XPS）分析证实，与未蚀刻的对应物相比，由于均匀的PDA沉积，表面蚀刻后表面N组成增加了约8.2%，从而增强了PDA沉积。双表面改性后表面化学性质和形态的变化促进了人成骨细胞（HOS）的附着和增殖，在PDA涂层蚀刻PLA（Et-PLAPDA）支架上细胞形态明显且铺展良好。此外，UMR-106细胞在蚀刻PLA（Et-PLA）和Et-PLAPDA上的成骨分化有显著改善，这突出了碱蚀刻介导的PDA沉积对改善PLA矿化的适用性。总体而言，目前的工作为改变支架表面组成、形貌、亲水性和粗糙度以调节局部细胞黏附，从而提高PLA的成骨潜力提供了思路。
