Chen Siqi, Zheng Yanyan, Fang Yan, Weng Yunxiang, Liu Haiqing, Chen Qinhui
College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, PR China.
College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, PR China; Fujian Provincial Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou 350007, PR China.
Colloids Surf B Biointerfaces. 2025 Mar;247:114453. doi: 10.1016/j.colsurfb.2024.114453. Epub 2024 Dec 10.
Hemostasis and subsequent anti-inflammatory measures are essential for wound healing in the human body following trauma or surgical procedures. Here, we try to use the dragging effect of a brush to prepare a Janus hydrogel with the least amount of bacteriostatic agent. The synthesized suspension of polyvinylbenzene-silica@quaternary ammonium salt (PDVB-SiO@NR) Janus particles (JNPs) was selected as ink and brush coated onto one side of a polyacrylic acid (PAA) hydrogel, resulting in Janus hydrogel (JNPs≌PAA). The anisotropic chemical composition, wetting properties, adhesion capabilities, and the obtained hemostatic performance of the Janus hydrogel were studied thoroughly. The hydrophilic PAA side promoted tissue adhesion, while the hydrophobic JNPs side exhibited antibacterial effects. The Janus hydrogel presented perfect hemostatic effect in vivo, owing to the procoagulant effect of the adhesive layer, negative charges, and blood-blocking properties of the hydrophobic layer. The presence of quaternary ammonium groups in the Janus hydrogel imparted strong antibacterial activity against E. coli and S. aureus. Furthermore, the Janus hydrogel showed a low hemolysis rate and high cell safety. This multifunctional hydrogel material holds great promise for wound treatment and expands the applications of hydrogel materials in the biomedical field.
止血及后续的抗炎措施对于人体创伤或手术后的伤口愈合至关重要。在此，我们尝试利用刷子的拖拽作用，以最少的抑菌剂制备一种Janus水凝胶。选择合成的聚苯乙烯 - 二氧化硅@季铵盐（PDVB - SiO@NR）Janus颗粒（JNPs）悬浮液作为墨水，并用刷子涂覆在聚丙烯酸（PAA）水凝胶的一侧，从而得到Janus水凝胶（JNPs≌PAA）。对Janus水凝胶的各向异性化学成分、润湿性、粘附能力以及所获得的止血性能进行了深入研究。亲水性的PAA侧促进组织粘附，而疏水性的JNPs侧表现出抗菌作用。Janus水凝胶在体内呈现出完美的止血效果，这归因于粘附层的促凝血作用、疏水层的负电荷以及阻血性能。Janus水凝胶中季铵基团的存在赋予了其对大肠杆菌和金黄色葡萄球菌强大的抗菌活性。此外，Janus水凝胶显示出低溶血率和高细胞安全性。这种多功能水凝胶材料在伤口治疗方面具有巨大潜力，并拓展了水凝胶材料在生物医学领域的应用。
