Lai Zhenquan, Wang Fucai, Cui Yishuang, Li Zhaofa, Lin Junsheng
College of Materials Science & Engineering, HuaQiao University, Amoy, Fujian, 361021, China; School of Medicine, Huaqiao University, Quanzhou, Fujian, 362021, China.
School of Medicine, Huaqiao University, Quanzhou, Fujian, 362021, China.
Talanta. 2025 Apr 1;285:127362. doi: 10.1016/j.talanta.2024.127362. Epub 2024 Dec 9.
Highly pathogenic coronaviruses have consistently threatened humanity, encompassing SARS-CoV, MERS-CoV, SARS-CoV-2 and others. Swift detection and accurate diagnosis play a crucial role in promptly identifying high-risk populations, enabling timely intervention, and effectively breaking the transmission chain to reduce casualties. However, the diagnostic "gold standard" reverse transcription-polymerase chain reaction (RT-PCR) failed to meet the overwhelming demand during the pandemic due to insufficient equipment and trained personnel, impeding the effective control of viral spread. Undoubtedly, there is an urgent need for the development of convenient, rapid, and sensitive point-of-care (POC) diagnostic technology. Gold nanoparticles (AuNPs) satisfy the substantial market demand for biosensors owing to their exceptional optical properties and stability. In this comprehensive review, we summarize the potential advantages of AuNPs in visual solution colorimetry and lateral flow assays (LFAs) for the diagnosis of COVID-19. We delve into the techniques for enhancing LFA signals, with the goal of increasing both detection sensitivity and specificity. Furthermore, we include the application of smartphones for unbiased and objective interpretation of results. The examples presented in this review are anticipated to inspire researchers in designing AuNPs biosensors to address current and potential outbreaks of infectious diseases in the future.
高致病性冠状病毒一直威胁着人类，包括严重急性呼吸综合征冠状病毒（SARS-CoV）、中东呼吸综合征冠状病毒（MERS-CoV）、严重急性呼吸综合征冠状病毒2（SARS-CoV-2）等。快速检测和准确诊断在及时识别高危人群、实现及时干预以及有效切断传播链以减少伤亡方面发挥着关键作用。然而，在疫情期间，诊断“金标准”逆转录聚合酶链反应（RT-PCR）由于设备和专业人员不足，无法满足巨大的需求，阻碍了对病毒传播的有效控制。毫无疑问，迫切需要开发便捷、快速且灵敏的即时检测（POC）诊断技术。金纳米颗粒（AuNPs）因其卓越的光学性质和稳定性，满足了生物传感器的巨大市场需求。在这篇综述中，我们总结了金纳米颗粒在用于新冠病毒（COVID-19）诊断的可视化溶液比色法和侧向流动分析（LFA）中的潜在优势。我们深入探讨增强LFA信号的技术，目标是提高检测的灵敏度和特异性。此外，我们还介绍了智能手机在无偏差和客观解读结果方面的应用。本综述中的实例有望激发研究人员设计金纳米颗粒生物传感器，以应对未来当前和潜在的传染病爆发。
