Deficiency of EXT1 and FGFR3 genes promotes chondrocyte differentiation, leading to the induction of osteochondroma formation.
作者信息
Zhang Hongrong, Tang Zhencun, Shen Shiying, Feng Lei, Qin Yunfa, Huang Liangchong, Chen Yanyan, Liu Yu, Wang Weihong
机构信息
Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming 650106, China; Yunnan Key Laboratory of Stomatology, Kunming 650106, China.
Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming 650106, China; Yunnan Key Laboratory of Stomatology, Kunming 650106, China; Department of Otolaryngology Head and Neck Surgery, 920th Hospital of Joint Logistics Support Force, PLA, China.
出版信息
Bone. 2025 Mar;192:117370. doi: 10.1016/j.bone.2024.117370. Epub 2024 Dec 13.
OBJECTIVE
This study aims to investigate the roles of the EXT1 and FGFR3 genes in the development of osteochondromas, focusing specifically on their potential interactions in chondrocyte proliferation, differentiation, and tumor formation.
METHODS
In vitro, the ATDC5 chondroprogenitor cell line was used to examine the effects of inactivation of both EXT1 and FGFR3. In vivo, a mouse model with dual gene knockout of Ext1 and Fgfr3 was constructed to further explore these genes' roles in tumor formation by observing the incidence and distribution patterns of osteochondromas.
RESULTS
The in vitro experiments demonstrated that ATDC5 cells with reduced expression of EXT1 and FGFR3 genes exhibited enhanced chondrogenic differentiation. In vivo, Fgfr3;Ext1 mice showed a significant incidence of osteochondromas (72.7 %), primarily located in the humerus, fibula, and tibia, while mice with a single heterozygous deletion did not display notable lesions.
CONCLUSION
The EXT1 and FGFR3 genes play crucial regulatory roles in the development of osteochondromas. Deficiencies in Ext1 and Fgfr3 can induce the formation of osteochondromas.
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