Ji Xiaolong, Yang Fan, Zhou Xiumei, Jia Wenqing, Zhu Xiaopei, Mu Jinyan, Wang Yanli, Zhang Yan, Mi Zhaorong, Zhang Shulin, Du Xiaohua, Song Xingshun
School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan 453000, China; Department of Genetics, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040, China.
School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, Henan 453000, China.
Int J Biol Macromol. 2025 Feb;288:138783. doi: 10.1016/j.ijbiomac.2024.138783. Epub 2024 Dec 13.
Cerasus humilis is a fruit tree with enormous potential economic value, and its fruit is rich in various bioactive substances. The basic helix loop helix (bHLH) gene family plays an important role in the biosynthesis of plant anthocyanins. However, there was no research on the ChbHLH gene family in C. humilis. In this study, 114 ChbHLH genes were identified from the C. humilis genome and divided into 17 subgroups. Then, evolutionary relationships, conserved motifs, gene structures, and cis-acting elements were analyzed. By predicting the interaction network between ChbHLH proteins and ChMYB1, it was found that ChbHLH44 (here named as ChEGL1) was located at the core of the interaction network. Further experiments revealed that ChEGL1 and ChMYB1 could interact with each other both in vivo and in vitro. In addition, ChEGL1 significantly increased the anthocyanin content in transgenic tomato plants. This study provides a comprehensive understanding of the ChbHLH gene family and supports further enrichment of the regulation mechanism of anthocyanin biosynthesis in C. humilis fruit.
欧李是一种具有巨大潜在经济价值的果树，其果实富含多种生物活性物质。基本螺旋-环-螺旋（bHLH）基因家族在植物花青素生物合成中起重要作用。然而，关于欧李中ChbHLH基因家族尚无研究。本研究从欧李基因组中鉴定出114个ChbHLH基因，并将其分为17个亚组。然后，分析了进化关系、保守基序、基因结构和顺式作用元件。通过预测ChbHLH蛋白与ChMYB1之间的相互作用网络，发现ChbHLH44（此处命名为ChEGL1）位于相互作用网络的核心。进一步实验表明，ChEGL1和ChMYB1在体内和体外均可相互作用。此外，ChEGL1显著提高了转基因番茄植株中的花青素含量。本研究为全面了解ChbHLH基因家族提供了依据，并为进一步丰富欧李果实花青素生物合成调控机制提供了支持。
