Mosca Barboza Camila, Santos Lima Juliana, Ferreira Gomes Bianca, Gonçalves Garcia Jaine, Pardo Souza Tatiane de Cássia, Mello Zamudio Raphaela, Rodrigues Chierato Maria Eduarda, De Novaes Oliveira Rafael, Carnieli Junior Pedro, Carlan da Silva Maria Cristina, Oliveira Fahl Willian, Mori Enio, Correa Scheffer Karin, Michel Roehe Paulo, Franco Ana Claudia, De Carvalho Ruthner Batista Helena Beatriz
Instituto Pasteur, São Paulo, SP, Brazil; Universidade Federal do ABC (UFABC), Santo André, SP, Brazil.
Instituto Pasteur, São Paulo, SP, Brazil.
Vet Ital. 2024 Jul 31;60(2). doi: 10.12834/VetIt.3427.23374.1.
Bats are mammals with vital role played in numerous ecosystem services, however bats can be important reservoirs or hosts for several microorganisms. Rabies is a zoonosis caused by Rabies lyssavirus (RABV) that affects the central nervous system (CNS) of all mammals, including bats and humans. The action of IFN-stimulated genes (ISGs) could be responsible for inhibiting different stages of the viral replication cycle. A major family of ISGs are the Interferon-induced proteins with tetrapeptide repeats (IFITs) and your action against infections caused by viruses from different families was proven. This study describes the expression of BatIFIT5 by RT-qPCR in different species of healthy and naturally infected with RABV bats. A total, of 36 bats were analyzed (18 positive and 18 negative for rabies) and 16 (44.44%) were positive for BatIFIT5. Here we analyzed fourteen species of bats with different eating and behavioral habits. Seven genetics lineages of RABV were evaluated and included in these 14 species of bats, no cases of RABV spillover were identified. In addition, we did not verified relationship between the bat species expression of BatIFIT5 and RABV. Many points about immunology of bats are unknown and here we analyzed one of these points.
蝙蝠是哺乳动物，在众多生态系统服务中发挥着至关重要的作用，然而蝙蝠可能是多种微生物的重要储存宿主。狂犬病是一种由狂犬病病毒（RABV）引起的人畜共患病，会影响包括蝙蝠和人类在内的所有哺乳动物的中枢神经系统（CNS）。干扰素刺激基因（ISG）的作用可能是抑制病毒复制周期的不同阶段。ISG的一个主要家族是具有四肽重复序列的干扰素诱导蛋白（IFIT），并且已证实其对不同病毒家族引起的感染具有作用。本研究通过RT-qPCR描述了BatIFIT5在健康和自然感染RABV的不同蝙蝠物种中的表达情况。总共分析了36只蝙蝠（18只狂犬病阳性和18只阴性），其中16只（44.44%）BatIFIT5呈阳性。在这里，我们分析了14种具有不同饮食和行为习惯的蝙蝠。评估了RABV的7个遗传谱系，这些谱系存在于这14种蝙蝠中，但未发现RABV溢出的情况。此外，我们未证实蝙蝠物种中BatIFIT5的表达与RABV之间的关系。关于蝙蝠免疫学的许多问题尚不清楚，在这里我们分析了其中一个问题。
