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用于牙科组织工程应用的甲基丙烯酰化明胶/海藻酸钠/纤维素纳米晶体油墨及3D打印

Gelatin Methacryloyl/Sodium Alginate/Cellulose Nanocrystal Inks and 3D Printing for Dental Tissue Engineering Applications.

作者信息

Li Huihua, Chen Shangsi, Dissanayaka Waruna Lakmal, Wang Min

机构信息

Applied Oral Sciences & Community Dental Care, Faculty of Dentistry, Prince Philip Dental Hospital, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong, Hong Kong.

Department of Mechanical Engineering Faculty of Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, Hong Kong.

出版信息

ACS Omega. 2024 Nov 25;9(49):48361-48373. doi: 10.1021/acsomega.4c06458. eCollection 2024 Dec 10.

DOI:10.1021/acsomega.4c06458
PMID:39676960
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11635503/
Abstract

In tissue engineering, developing suitable printing inks for fabricating hydrogel scaffolds via 3D printing is of high importance and requires extensive investigation. Currently, gelatin methacryloyl (GelMA)-based inks have been widely used for the construction of 3D-printed hydrogel scaffolds and cell-scaffold constructs for human tissue regeneration. However, many studies have shown that GelMA inks at low polymer concentrations had poor printability, and printed structures exhibited inadequate fidelity. In the current study, new viscoelastic inks composed of gelatin methacryloyl (GelMA), sodium alginate (Alg), and cellulose nanocrystal (CNC) were formulated and investigated, with CNC being used to improve the printability of inks and the fidelity of printed hydrogel structures and Alg being used to form ionically cross-linking polymer networks to enhance the mechanical strength of printed hydrogel structures. Rheological results showed that GelMA/Alg/CNC inks with different Alg-to-CNC ratios possessed good shear-thinning behavior, indicating that GelMA/Alg/CNC inks were suitable for 3D printing. The quantitative evaluation of printability and fidelity showed that a high concentration of CNC improved the printability of GelMA/Alg/CNC inks and concurrently promoted the fidelity of printed GelMA/Alg/CNC hydrogels. On the other hand, compression tests showed that a high concentration of Alg could enhance the mechanical strength of GelMA/Alg/CNC hydrogels due to the increase in cross-link density. Furthermore, GelMA/Alg/CNC hydrogels exhibited good biocompatibility and could promote the proliferation of human dental pulp stem cells (hDPSCs), suggesting their great potential in dental tissue engineering.

摘要

在组织工程中，开发适用于通过3D打印制造水凝胶支架的打印墨水至关重要，需要进行广泛研究。目前，基于甲基丙烯酰化明胶（GelMA）的墨水已被广泛用于构建3D打印水凝胶支架和用于人类组织再生的细胞-支架构建体。然而，许多研究表明，低聚合物浓度的GelMA墨水可打印性差，打印结构的保真度不足。在本研究中，配制并研究了由甲基丙烯酰化明胶（GelMA）、海藻酸钠（Alg）和纤维素纳米晶体（CNC）组成的新型粘弹性墨水，其中CNC用于改善墨水的可打印性和打印水凝胶结构的保真度，Alg用于形成离子交联聚合物网络以增强打印水凝胶结构的机械强度。流变学结果表明，不同Alg与CNC比例的GelMA/Alg/CNC墨水具有良好的剪切变稀行为，表明GelMA/Alg/CNC墨水适用于3D打印。可打印性和保真度的定量评估表明，高浓度的CNC改善了GelMA/Alg/CNC墨水的可打印性，同时提高了打印的GelMA/Alg/CNC水凝胶的保真度。另一方面，压缩测试表明，由于交联密度的增加，高浓度的Alg可以增强GelMA/Alg/CNC水凝胶的机械强度。此外，GelMA/Alg/CNC水凝胶表现出良好的生物相容性，并能促进人牙髓干细胞（hDPSCs）的增殖，表明它们在牙科组织工程中具有巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/2456ad903372/ao4c06458_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/37d47f08ff50/ao4c06458_0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/4e3d7b33073f/ao4c06458_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/c449f7c02b56/ao4c06458_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/6b46b689776c/ao4c06458_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/bdedb0178d92/ao4c06458_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/f1b3d16d79c7/ao4c06458_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/2456ad903372/ao4c06458_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/37d47f08ff50/ao4c06458_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/d23343d0eb9e/ao4c06458_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/352813c04814/ao4c06458_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/f9998830c275/ao4c06458_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/4e3d7b33073f/ao4c06458_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/c449f7c02b56/ao4c06458_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/6b46b689776c/ao4c06458_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/bdedb0178d92/ao4c06458_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/f1b3d16d79c7/ao4c06458_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe13/11635503/2456ad903372/ao4c06458_0010.jpg

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