通过12-(9-蒽酰基)硬脂酸的荧光猝灭测量脂质体中泛醌的横向扩散系数。
Measurement of the lateral diffusion coefficients of ubiquinones in lipid vesicles by fluorescence quenching of 12-(9-anthroyl)stearate.
作者信息
Fato R, Battino M, Parenti Castelli G, Lenaz G
出版信息
FEBS Lett. 1985 Jan 7;179(2):238-42. doi: 10.1016/0014-5793(85)80526-3.
PMID:3967755
Abstract
The lateral diffusion coefficients of some ubiquinone homologues have been measured in phospholipid vesicles exploiting the fluorescence quenching of the probe 12-(9-anthroyl)stearate by the quinones. Diffusion coefficients higher than 10(-6) cm2 X s-1 have been found at 25 degrees C, compatible with the localization of the ubiquinones in the low-viscosity midplane region of the bilayer.
摘要
利用醌类对探针12-(9-蒽酰基)硬脂酸酯的荧光猝灭作用，已在磷脂囊泡中测量了一些泛醌同系物的侧向扩散系数。在25℃下发现扩散系数高于10^(-6) cm²·s⁻¹，这与泛醌在双层低粘度中间平面区域的定位相一致。
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