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心脏直视手术中偶然获取的人胸骨骨髓的采集与克隆生长。

Acquisition and clonal growth of human sternal bone marrow obtained incidental to open-heart surgery.

作者信息

Wittberg K L, De Wall R A, Murphy M J

出版信息

Exp Cell Biol. 1985;53(1):41-5. doi: 10.1159/000163294.

DOI:10.1159/000163294
PMID:3967795
Abstract

The establishment of a convenient method of acquiring ample human bone marrow and the successful growth of hematopoietic precursor cells in standard methylcellulose assay is described. Human sternal marrow curettings were obtained at open-heart surgery. Growth of colonies derived from CFU-gm, BFU-e, and CFU-e was successful. Comparison of the CFU-gm colony production between this and prior studies indicates a consistent mean and range of growth. These results document this to be an efficient source of normal human marrow.

摘要

本文描述了一种获取充足人骨髓的便捷方法的建立,以及造血前体细胞在标准甲基纤维素试验中成功生长的情况。人胸骨骨髓刮屑取自心脏直视手术。源自粒系集落形成单位(CFU-gm)、爆式红系集落形成单位(BFU-e)和红系集落形成单位(CFU-e)的集落生长成功。此次研究与之前研究的CFU-gm集落产量比较显示,生长的平均水平和范围一致。这些结果证明这是正常人类骨髓的一个有效来源。

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