Ahlstedt S, Björkstén B, Holgersson M, Nygren H, Smedegård G
Int Arch Allergy Appl Immunol. 1985;76(1):52-7. doi: 10.1159/000233661.
Relations between the appearance of various components of the immune response were analyzed in two groups of rats sensitized by aerosol and subcutaneous injections in the neck region, respectively. The relations were expressed by Spearman rank correlation coefficient and studied by cluster analysis. In the aerosol-sensitized animals, there was a close association between IgA and IgG antibody levels in bronchial fluid and these in turn were related to IgG levels in serum and more loosely to IgE levels in bronchial fluid. There was an apparent association between IgE antibody formation and mast cell maturation in cultures of regional lymph node cells and the appearance of mucous cells in the lungs. These variables seemed associated with spontaneous cell proliferation in vitro and the numbers of mast cells in the lungs. This indicates that local stimulation with antigen induces local immune responses and immune-mediated migration of cells. In subcutaneously sensitized animals, formation of IgG antibodies in vitro seemed related to the stimulated proliferation of regional lymph node cells. The levels of IgG and IgE antibodies in bronchial fluid and in serum also appeared to be related. Unlike the findings in aerosol-sensitized animals there was no apparent relation between the differentiation of mast cells and mucous cells. This was possibly due to lack of immune-mediated antigen-induced cell migration. The different immune response patterns in aerosol and subcutaneously sensitized rats should be considered when studies are designed aiming to explore the pathogenesis of allergic inflammatory diseases. The findings also indicate that the various parameters of immunity are more closely related in aerosol than in subcutaneously immunized animals.
分别对两组经气溶胶致敏和颈部皮下注射致敏的大鼠的免疫反应各组分的表现之间的关系进行了分析。这些关系用斯皮尔曼等级相关系数表示，并通过聚类分析进行研究。在经气溶胶致敏的动物中，支气管液中的IgA和IgG抗体水平之间存在密切关联，而这些又依次与血清中的IgG水平相关，与支气管液中的IgE水平的相关性则较弱。在局部淋巴结细胞培养物中，IgE抗体形成与肥大细胞成熟之间以及肺中黏液细胞的出现之间存在明显关联。这些变量似乎与体外自发细胞增殖以及肺中肥大细胞的数量有关。这表明抗原的局部刺激可诱导局部免疫反应和细胞的免疫介导迁移。在经皮下致敏的动物中，体外IgG抗体的形成似乎与局部淋巴结细胞的刺激增殖有关。支气管液和血清中IgG和IgE抗体的水平似乎也有关联。与经气溶胶致敏的动物的发现不同，肥大细胞和黏液细胞的分化之间没有明显关联。这可能是由于缺乏免疫介导的抗原诱导细胞迁移。在设计旨在探索过敏性炎症性疾病发病机制的研究时，应考虑气溶胶致敏和皮下致敏大鼠中不同的免疫反应模式。这些发现还表明，与皮下免疫的动物相比，气溶胶免疫动物的各种免疫参数之间的相关性更强。
