胡萝卜软腐欧文氏菌胡萝卜软腐亚种和黑胫亚种的高效转化
Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.
作者信息
Hinton J C, Perombelon M C, Salmond G P
出版信息
J Bacteriol. 1985 Feb;161(2):786-8. doi: 10.1128/jb.161.2.786-788.1985.
Abstract
We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colonies per micrograms of plasmid DNA. The nature of these transformants was confirmed by plasmid analysis. ColE1-based plasmids make potentially useful cloning vectors for the study of genes involved in the pathogenesis of this species.
摘要
我们采用了Hanahan方法（D. Hanahan，《分子生物学杂志》166:557 - 580，1983年）的改良版本，用质粒pBR322、pBR325和pAT153转化胡萝卜软腐欧文氏菌胡萝卜软腐亚种和胡萝卜软腐欧文氏菌黑胫亚种。转化频率为每微克质粒DNA产生1×10²至4×10⁴个菌落。通过质粒分析证实了这些转化体的性质。基于ColE1的质粒成为研究该物种致病相关基因的潜在有用克隆载体。
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