• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

胡萝卜软腐欧文氏菌胡萝卜软腐亚种和黑胫亚种的高效转化

Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.

作者信息

Hinton J C, Perombelon M C, Salmond G P

出版信息

J Bacteriol. 1985 Feb;161(2):786-8. doi: 10.1128/jb.161.2.786-788.1985.

DOI:10.1128/jb.161.2.786-788.1985
PMID:3968041
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214956/
Abstract

We used a modified version of the method of Hanahan (D. Hanahan, J. Mol. Biol. 166:557-580, 1983) to transform Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica with the plasmids pBR322, pBR325, and pAT153. The transformation frequency ranged from 1 X 10(2) to 4 X 10(4) colonies per micrograms of plasmid DNA. The nature of these transformants was confirmed by plasmid analysis. ColE1-based plasmids make potentially useful cloning vectors for the study of genes involved in the pathogenesis of this species.

摘要

我们采用了Hanahan方法（D. Hanahan，《分子生物学杂志》166:557 - 580，1983年）的改良版本，用质粒pBR322、pBR325和pAT153转化胡萝卜软腐欧文氏菌胡萝卜软腐亚种和胡萝卜软腐欧文氏菌黑胫亚种。转化频率为每微克质粒DNA产生1×10²至4×10⁴个菌落。通过质粒分析证实了这些转化体的性质。基于ColE1的质粒成为研究该物种致病相关基因的潜在有用克隆载体。

相似文献

1
Efficient transformation of Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.胡萝卜软腐欧文氏菌胡萝卜软腐亚种和黑胫亚种的高效转化
J Bacteriol. 1985 Feb;161(2):786-8. doi: 10.1128/jb.161.2.786-788.1985.
2
pULB113, an RP4::mini-Mu plasmid, mediates chromosomal mobilization and R-prime formation in Erwinia amylovora, Erwinia chrysanthemi, and subspecies of Erwinia carotovora.pULB113是一种RP4::mini-Mu质粒，可介导梨火疫病菌、菊欧文氏菌和胡萝卜软腐欧文氏菌亚种中的染色体转移和R因子形成。
Appl Environ Microbiol. 1985 Jul;50(1):1-9. doi: 10.1128/aem.50.1.1-9.1985.
3
Characterization of transposon insertion out- mutants of Erwinia carotovora subsp. carotovora defective in enzyme export and of a DNA segment that complements out mutations in E. carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Erwinia chrysanthemi.胡萝卜软腐欧文氏菌胡萝卜软腐亚种转座子插入外排突变体的特性分析，这些突变体在酶输出方面存在缺陷，以及一个能互补胡萝卜软腐欧文氏菌胡萝卜软腐亚种、黑腐欧文氏菌亚种和菊欧文氏菌外排突变的DNA片段。
J Bacteriol. 1990 Jun;172(6):2970-8. doi: 10.1128/jb.172.6.2970-2978.1990.
4
Isolation by genomic subtraction of DNA probes specific for Erwinia carotovora subsp. atroseptica.通过基因组消减分离胡萝卜软腐欧文氏菌黑腐亚种特异性DNA探针。
Appl Environ Microbiol. 1994 Jan;60(1):298-306. doi: 10.1128/aem.60.1.298-306.1994.
5
[Expression of pectate lyase genes of Erwinia carotovora subsp. carotovora 17A and Erwinia carotovora subsp. atroseptica 36A in Erwinia carotovor substp. atroseptica 36A cells].[胡萝卜软腐欧文氏菌胡萝卜软腐亚种17A和黑胫亚种36A果胶酸裂解酶基因在黑胫亚种36A细胞中的表达]
Mol Gen Mikrobiol Virusol. 1994 Nov-Dec(6):23-7.
6
Transposon Tn5 mutagenesis in Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.胡萝卜软腐欧文氏菌胡萝卜软腐亚种和黑胫亚种中的转座子Tn5诱变
J Bacteriol. 1984 Mar;157(3):809-14. doi: 10.1128/jb.157.3.809-814.1984.
7
Rapid identification and differentiation of the soft rot erwinias by 16S-23S intergenic transcribed spacer-PCR and restriction fragment length polymorphism analyses.通过16S-23S基因间转录间隔区PCR和限制性片段长度多态性分析快速鉴定和区分软腐欧文氏菌。
Appl Environ Microbiol. 2001 Sep;67(9):4070-6. doi: 10.1128/AEM.67.9.4070-4076.2001.
8
[Method for transformation of Erwinia carotovora subsp. carotovora cells by plasmid pECL18].[利用质粒pECL18转化胡萝卜软腐欧文氏菌胡萝卜软腐亚种细胞的方法]
Mikrobiol Z. 2007 Sep-Oct;69(5):35-41.
9
Regulation of the production of extracellular pectinase, cellulase, and protease in the soft rot bacterium Erwinia carotovora subsp. carotovora: evidence that aepH of E. carotovora subsp. carotovora 71 activates gene expression in E. carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Escherichia coli.胡萝卜软腐欧文氏菌胡萝卜软腐亚种中细胞外果胶酶、纤维素酶和蛋白酶产生的调控：胡萝卜软腐欧文氏菌胡萝卜软腐亚种71的aepH激活胡萝卜软腐欧文氏菌胡萝卜软腐亚种、黑腐亚种以及大肠杆菌中基因表达的证据。
Appl Environ Microbiol. 1994 Sep;60(9):3150-9. doi: 10.1128/aem.60.9.3150-3159.1994.
10
Immunomagnetic separation of Erwinia carotovora subsp. atroseptica from potato peel extracts to improve detection sensitivity on a crystal violet pectate medium or by PCR.从马铃薯皮提取物中免疫磁分离胡萝卜软腐欧文氏菌黑胫亚种，以提高在结晶紫果胶酸盐培养基上或通过聚合酶链式反应的检测灵敏度。
J Appl Bacteriol. 1996 May;80(5):487-95. doi: 10.1111/j.1365-2672.1996.tb03247.x.

引用本文的文献

1
Injectisome T3SS subunits as potential chaperones in the extracellular export of Pectobacterium carotovorum subsp. carotovorum bacteriocins Carocin S1 and Carocin S3 secreted via flagellar T3SS.注入体 T3SS 亚基作为果胶杆菌亚种胡萝卜软腐欧文氏菌细菌素 Carocin S1 和 Carocin S3 通过鞭毛 T3SS 分泌的细胞外出口的潜在伴侣蛋白。
BMC Microbiol. 2021 Dec 15;21(1):345. doi: 10.1186/s12866-021-02405-w.
2
Microbial population dynamics in response to Pectobacterium atrosepticum infection in potato tubers.马铃薯块茎中响应黑胫果胶杆菌感染的微生物种群动态
Sci Rep. 2015 Jun 29;5:11606. doi: 10.1038/srep11606.
3
GSL2 over-expression confers resistance to Pectobacterium atrosepticum in potato.GSL2 过表达赋予马铃薯对果胶杆菌的抗性。
Theor Appl Genet. 2014 Mar;127(3):677-89. doi: 10.1007/s00122-013-2250-2. Epub 2013 Dec 27.
4
Role and regulation of the Flp/Tad pilus in the virulence of Pectobacterium atrosepticum SCRI1043 and Pectobacterium wasabiae SCC3193.Flp/Tad 菌毛在果胶杆菌 SCRI1043 和 Pectobacterium wasabiae SCC3193 毒力中的作用和调控。
PLoS One. 2013 Sep 9;8(9):e73718. doi: 10.1371/journal.pone.0073718. eCollection 2013.
5
AepA of Pectobacterium is not involved in the regulation of extracellular plant cell wall degrading enzymes production.果胶杆菌 AepA 不参与调控胞外植物细胞壁降解酶的产生。
Mol Genet Genomics. 2010 Jun;283(6):541-9. doi: 10.1007/s00438-010-0540-9. Epub 2010 Apr 13.
6
Extracellular secretion of Carocin S1 in Pectobacterium carotovorum subsp. carotovorum occurs via the type III secretion system integral to the bacterial flagellum.胡萝卜软腐果胶杆菌胡萝卜软腐亚种中Carocin S1的细胞外分泌通过与细菌鞭毛整合的III型分泌系统进行。
BMC Microbiol. 2009 Aug 27;9:181. doi: 10.1186/1471-2180-9-181.
7
Distinguishing bacterial pathogens of potato using a genome-wide microarray approach.使用全基因组微阵列方法区分马铃薯的细菌病原体。
Mol Plant Pathol. 2008 Sep;9(5):705-17. doi: 10.1111/j.1364-3703.2008.00482.x.
8
Cloning and expression of the Erwinia carotovora subsp. carotovora gene encoding the low-molecular-weight bacteriocin carocin S1.胡萝卜软腐欧文氏菌胡萝卜软腐亚种编码低分子量细菌素胡萝卜菌素S1的基因的克隆与表达
J Bacteriol. 2007 Jan;189(2):620-6. doi: 10.1128/JB.01090-06. Epub 2006 Oct 27.
9
Two Genomic Regions Involved in Catechol Siderophore Production by Erwinia carotovora.两个参与产碱果胶酸钙细菌儿茶酚 siderophore 生产的基因组区域。
Appl Environ Microbiol. 1994 Feb;60(2):662-9. doi: 10.1128/aem.60.2.662-669.1994.
10
Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors.肠杆菌属植物病原菌胡萝卜软腐欧文氏菌黑腐亚种的基因组序列及毒力因子特性分析
Proc Natl Acad Sci U S A. 2004 Jul 27;101(30):11105-10. doi: 10.1073/pnas.0402424101. Epub 2004 Jul 19.

本文引用的文献

1
Trans-complementable copy-number mutants of plasmid ColE1.质粒ColE1的反式可互补拷贝数突变体
Nature. 1980 Jan 10;283(5743):216-8. doi: 10.1038/283216a0.
2
Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA.球形红假单胞菌的质粒DNA遗传转化
J Bacteriol. 1982 Oct;152(1):89-97. doi: 10.1128/jb.152.1.89-97.1982.
3
Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
4
Transposon Tn5 mutagenesis in Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica.胡萝卜软腐欧文氏菌胡萝卜软腐亚种和黑胫亚种中的转座子Tn5诱变
J Bacteriol. 1984 Mar;157(3):809-14. doi: 10.1128/jb.157.3.809-814.1984.
5
Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.专用质粒克隆载体。II. 广泛宿主范围、高拷贝数、源自RSF1010的载体以及用于假单胞菌基因克隆的宿主-载体系统。
Gene. 1981 Dec;16(1-3):237-47. doi: 10.1016/0378-1119(81)90080-9.
6
Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome.广宿主范围载体和基因文库的构建：铜绿假单胞菌PAO染色体的自克隆
J Bacteriol. 1982 Apr;150(1):60-9. doi: 10.1128/jb.150.1.60-69.1982.
7
A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
8
Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.超螺旋脱氧核糖核酸-蛋白质松弛复合体的性质及松弛事件的链特异性
Biochemistry. 1970 Oct 27;9(22):4428-40. doi: 10.1021/bi00824a026.
9
Frozen-thawed bacteria as recipients of isolated coliphage DNA.
Biochim Biophys Acta. 1972 Oct 27;281(3):319-23. doi: 10.1016/0005-2787(72)90444-3.
10
Genetic studies with heteroduplex DNA of bacteriophage fl. Asymmetric segregation, base correction and implications for the mechanism of genetic recombination.用噬菌体fl的异源双链DNA进行的遗传学研究。不对称分离、碱基校正及其对基因重组机制的意义。
J Mol Biol. 1975 Aug 15;96(3):495-509. doi: 10.1016/0022-2836(75)90175-8.