在感染性角膜炎的体外角膜交联模型中紫外线A和绿光的透射率。

Transmission rates of UV-A and green light in an ex vivo corneal crosslinking model for infectious keratitis.

作者信息

Lu Nan-Ji, Meier Philipp, Reina Giacomo, Aydemir M Enes, Eitner Stephanie, Koliwer-Brandl Hendrik, Egli Adrian, Kissling Vera, Wick Peter, Hafezi Farhad

机构信息

From the Department of Ophthalmology, West China Hospital Sichuan University, Chengdu, China (Lu); ELZA Institute, Dietikon, Switzerland (Lu, Aydemir, Hafezi); Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland (Lu, Koliwer-Brandl, Egli); Particles-Biology Interactions Laboratory, Empa - Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland (Meier, Reina, Eitner, Kissling, Wick); Ocular Cell Biology Group, Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Zurich, Switzerland (Hafezi); Department of Ophthalmology at New York University Grossman School of Medicine, New York University, New York, New York; Faculty of Medicine, University of Geneva, Geneva, Switzerland (Hafezi); Department of Ophthalmology, Wenzhou Medical University, Wenzhou, China (Hafezi).

出版信息

J Cataract Refract Surg. 2025 Mar 1;51(3):249-256. doi: 10.1097/j.jcrs.0000000000001599.

DOI:10.1097/j.jcrs.0000000000001599

PMID:39680566

Abstract

PURPOSE

To investigate the light transmission (LT) of UV-A and green light through infected corneas saturated with riboflavin or rose bengal in an ex vivo porcine model for infectious keratitis.

SETTING

University of Zurich, Zurich, Switzerland, and Empa, St. Gallen, Switzerland.

DESIGN

Laboratory study.

METHODS

Ex vivo porcine eyes (n = 162) were divided into 3 groups: control eyes, eyes infected with Staphylococcus aureus , and eyes infected with Pseudomonas aeruginosa . Corneas remained either uninfected or were infected with S. aureus and P. aeruginosa and were either left untreated or were instilled with 0.1% riboflavin or 0.1% rose bengal. Corneal buttons were prepared, and corneal LT was measured at 365 nm and 522 nm using a spectrophotometer. LT values were calculated and compared. Transmission electron microscopy (TEM) was used to visualize structural damage and bacteria within infected corneas.

RESULTS

Riboflavin-saturated corneas infected by S. aureus or P. aeruginosa (LT = 0.77% [0.41-1.87] and 0.81% [0.23, 1.46]) exhibited 3.18-fold and 3.02-fold lower LT values, respectively, than uninfected corneas (LT = 2.45% [2.15, 5.89]) (both P -values < 0.001). No LT difference was found between rose bengal-saturated corneas infected by S. aureus or P. aeruginosa and uninfected corneas (all LT values = 0.01% [0.01-0.01]; both P -values = 0.08). TEM showed bacteria on corneal stroma borders and occasionally inside the stroma.

CONCLUSIONS

The results indicate that the amount of light arriving at the corneal endothelium is substantially reduced in infected corneas. The total fluence of clinical photoactivated chromophore for keratitis corneal crosslinking protocols can be safely increased substantially while maintaining a low risk of corneal endothelial damage.

摘要

目的

在感染性角膜炎的离体猪模型中，研究紫外线A和绿光透过用核黄素或孟加拉玫瑰红饱和处理的感染角膜的光透射率（LT）。

设置

瑞士苏黎世大学和瑞士圣加仑的瑞士联邦材料科学与技术实验室。

设计

实验室研究。

方法

将离体猪眼（n = 162）分为3组：对照眼、感染金黄色葡萄球菌的眼和感染铜绿假单胞菌的眼。角膜要么未感染，要么感染金黄色葡萄球菌和铜绿假单胞菌，且要么不进行处理，要么滴入0.1%核黄素或0.1%孟加拉玫瑰红。制备角膜纽扣片，使用分光光度计在365纳米和522纳米处测量角膜LT。计算并比较LT值。使用透射电子显微镜（TEM）观察感染角膜内的结构损伤和细菌。

结果

被金黄色葡萄球菌或铜绿假单胞菌感染的核黄素饱和角膜（LT分别为0.77% [0.41 - 1.87]和0.81% [0.23, 1.46]）的LT值分别比未感染角膜（LT = 2.45% [2.15, 5.89]）低3.18倍和3.02倍（P值均< 0.001）。被金黄色葡萄球菌或铜绿假单胞菌感染的孟加拉玫瑰红饱和角膜与未感染角膜之间未发现LT差异（所有LT值 = 0.01% [0.01 - 0.01]；P值均 = 0.08）。TEM显示细菌位于角膜基质边界，偶尔也在基质内部。