Li Hansheng, Cheng Chunzhen, Chen Mulan, Li Yuqing, Li Jinkun, Ye Wei, Sun Gang
Fujian Provincial Key Laboratory of Bamboo Resources Development and Utilization, Sanming University, No. 25 Jingdong, Sanming City, Fujian Province, 365004, China.
College of Horticulture, Shanxi Agricultural University, No. 81 Longcheng Street, Xiaodian District, Taiyuan City, Shanxi Province, 030801, China.
Tree Physiol. 2024 Dec 16;44(12). doi: 10.1093/treephys/tpae144.
Anoectochilus roxburghii (Wall.) Lind. has significant medicinal and economic value, and the social demand for this species is increasing annually. Laser light sources have different luminescent mechanisms compared with ordinary light sources and are also important factors regulating the synthesis of functional metabolites in A. roxburghii. However, the regulatory mechanism through which A. roxburghii responds to blue laser light has not been investigated. Previous studies have shown that blue-laser treatment results in more red leaves than blue- or white-light treatment. Here, the differences in the effects of laser treatment on A. roxburghii were analyzed by transcriptome sequencing. Gene Ontology analysis revealed that the membranes, calcium ion binding, brassinosteroid-mediated signaling pathway and response to salicylic acid play important roles in the response of A. roxburghii to blue laser light. Kyoto Encyclopedia of Genes and Genomes analysis revealed the involvement of multiple pathways in the response to blue-laser treatment, and among these, beta biosynthesis, flavone and flavonol biosynthesis, thiamine metabolism, limonene and pinene biosynthesis, and peroxisomes play core roles. Cytoscape interaction analysis of the differentially expressed miRNA targets indicated that novel_miR_66, novel_miR_78 and novel_miR_212 were most likely involved in the effect of blue laser light on A. roxburghii. Metabolic content measurements showed that blue laser light increased the beet red pigment, thiamine, total flavonoid and limonene contents, and qPCR analysis confirmed that novel_miR_21, novel_miR_66, novel_miR_188 and novel_miR_194 might participate in the blue-laser signaling network through their target genes and thereby regulate the functional metabolite accumulation in A. roxburghii. This study provides a scientific basis for high-yield A. roxburghii production.
金线莲具有显著的药用和经济价值，社会对该物种的需求逐年增加。与普通光源相比，激光光源具有不同的发光机制，也是调节金线莲中功能代谢物合成的重要因素。然而，金线莲对蓝光激光的响应调控机制尚未得到研究。先前的研究表明，与蓝光或白光处理相比，蓝光激光处理会产生更多的红叶。在此，通过转录组测序分析了激光处理对金线莲影响的差异。基因本体分析表明，膜、钙离子结合、油菜素内酯介导的信号通路以及对水杨酸的响应在金线莲对蓝光激光的响应中起重要作用。京都基因与基因组百科全书分析表明，多条通路参与了对蓝光激光处理的响应，其中，β-生物合成、黄酮和黄酮醇生物合成、硫胺素代谢、柠檬烯和蒎烯生物合成以及过氧化物酶体发挥核心作用。对差异表达的miRNA靶标的Cytoscape相互作用分析表明，novel_miR_66、novel_miR_78和novel_miR_212最有可能参与蓝光激光对金线莲的影响。代谢含量测定表明，蓝光激光增加了甜菜红色素、硫胺素、总黄酮和柠檬烯的含量，qPCR分析证实，novel_miR_21、novel_miR_66、novel_miR_188和novel_miR_194可能通过其靶基因参与蓝光激光信号网络，从而调节金线莲中功能代谢物的积累。本研究为金线莲的高产生产提供了科学依据。
