Stevanović Marija, Tagkalidou Niki, Multisanti Cristiana Roberta, Pujol Sergi, Aljabasini Ouwais, Prats Eva, Faggio Caterina, Porta Josep Maria, Barata Carlos, Raldúa Demetrio
Institute of Pesticides and Environmental Protection, Banatska 31b, 11080 Belgrade, Serbia.
Institute for Environmental Assessment and Water Research (IDAEA-CSIC), Jordi Girona, 18, 08034 Barcelona, Spain.
Sci Total Environ. 2025 Jan 1;958:178028. doi: 10.1016/j.scitotenv.2024.178028. Epub 2024 Dec 15.
The acoustic startle response (ASR) is leaded by a sudden and intense acoustic stimulus. ASR has several forms of plasticity, including habituation and sensorimotor gating. Although ASR and its plasticity have been intensively studied in zebrafish (Danio rerio) larvae, information in adult zebrafish is still very scarce. In this manuscript we present Zebra_K, a new automated high-content kinematic analysis platform for assessing ASR, its habituation and prepulse inhibition (PPI), a quantitative measure of sensorimotor gating, in adult zebrafish. The analysis of the kinematic parameters of ASR in adult zebrafish has shown a single response wave consistent with the short-latency C-bend described in zebrafish larvae. Moreover, protocols have been designed and validated in Zebra_K for the analysis of sensitivity, habituation and PPI of this response. Then, the effect of the time of day and the gender on zebrafish ASR plasticity has been analyzed for the first time. Females exhibited higher responsiveness and a lower habituation and PPI than males, a result consistent with the gender effect described in other animal models and in humans. This platform has also been used to determine the effect of a pharmacological modulators of ASR plasticity, the NMDA-receptor antagonist ketamine. As described in other animal models, ketamine increased the responsiveness to the acoustic stimuli, decreasing habituation and leading to complete abolition of PPI. These results enhance the interest of using adult zebrafish to assess the potential effect of environmental pollutants on ASR plasticity.
听觉惊吓反应（ASR）由突然而强烈的听觉刺激引发。ASR具有多种形式的可塑性，包括习惯化和感觉运动门控。尽管在斑马鱼（Danio rerio）幼体中对ASR及其可塑性进行了深入研究，但成年斑马鱼方面的信息仍然非常稀少。在本论文中，我们介绍了Zebra_K，这是一个用于评估成年斑马鱼ASR、其习惯化和前脉冲抑制（PPI，感觉运动门控的一种定量测量）的新型自动化高内涵运动分析平台。对成年斑马鱼ASR运动学参数的分析显示出一个单一的反应波，与斑马鱼幼体中描述的短潜伏期C型弯曲一致。此外，已经在Zebra_K中设计并验证了用于分析该反应的敏感性、习惯化和PPI的实验方案。然后，首次分析了一天中的时间和性别对斑马鱼ASR可塑性的影响。雌性比雄性表现出更高的反应性、更低的习惯化和PPI，这一结果与其他动物模型和人类中描述的性别效应一致。该平台还被用于确定ASR可塑性的药理学调节剂——NMDA受体拮抗剂氯胺酮的作用。正如在其他动物模型中所描述的，氯胺酮增加了对听觉刺激的反应性，降低了习惯化并导致PPI完全消失。这些结果提高了使用成年斑马鱼评估环境污染物对ASR可塑性潜在影响的关注度。
