Wong S Y, Kariks J, Evans R A, Dunstan C R, Hills E
J Bone Joint Surg Am. 1985 Feb;67(2):274-83.
We studied the effect of age on bone composition and osteocyte viability in femoral heads from fifty-one subjects. The assessment included determination of: bone volume, ash weight, calcium, and phosphorus content; osteocyte viability in fresh sections stained for lactate dehydrogenase activity; microfractures in fresh sections after removal of marrow elements; bone area, the presence of metabolic bone disease, and the histology of microfractures in embedded calcified sections; and the extent of trabecular microfractures. Bone area and numbers of microfractures were also assessed in eight elderly hip-fracture patients. Bone volume decreased with age, but there was considerable variation in each age group, and no significant difference between men and women. Ash weight and the bone content of calcium and phosphorus also decreased with age, but were constant if corrected for bone volume. Almost all osteocytes were viable in subjects who were younger than twenty-five years, and thereafter viability progressively decreased to a mean of 74 per cent in the eighth decade of life. There was a significant negative correlation between osteocyte viability and age. There was no evidence of metabolic bone disease in any patient. The numbers of microfractures increased with age and correlated negatively with bone viability (r = -0.31, p less than 0.05); in simple linear correlation a relationship between bone area and microfractures could not be demonstrated but in multiple linear correlation, after the inclusion of bone viability, there was an additional negative correlation between numbers of microfractures and bone area (p less than 0.005). Bone area and numbers of microfractures in hip-fracture patients were similar to those in age-matched controls.
我们研究了年龄对51名受试者股骨头骨成分和骨细胞活力的影响。评估内容包括：骨体积、灰重、钙和磷含量；新鲜切片中乳酸脱氢酶活性染色的骨细胞活力；去除骨髓成分后新鲜切片中的微骨折；钙化包埋切片中的骨面积、代谢性骨病的存在情况以及微骨折的组织学；以及小梁微骨折的程度。还对8名老年髋部骨折患者的骨面积和微骨折数量进行了评估。骨体积随年龄增长而下降，但各年龄组存在相当大的差异，且男女之间无显著差异。灰重以及钙和磷的骨含量也随年龄增长而下降，但如果根据骨体积进行校正则保持不变。几乎所有25岁以下受试者的骨细胞都具有活力，此后活力逐渐下降，在80岁时平均降至74%。骨细胞活力与年龄之间存在显著负相关。任何患者均无代谢性骨病的证据。微骨折数量随年龄增长而增加，且与骨活力呈负相关（r = -0.31，p < 0.05）；在简单线性相关中，无法证明骨面积与微骨折之间的关系，但在多元线性相关中，纳入骨活力后，微骨折数量与骨面积之间还存在额外的负相关（p < 0.005）。髋部骨折患者的骨面积和微骨折数量与年龄匹配的对照组相似。
