Khalil Heba M A, Eid Walaa A M, El-Nablaway Mohammad, El Nashar Eman Mohamad, Al-Tarish Jaber Saad, El Henafy Hanan M A
Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Food Science and Technology Department, Faculty of Agriculture, New Valley University, El-Kharga City, Egypt.
Sci Rep. 2024 Dec 16;14(1):30480. doi: 10.1038/s41598-024-80197-5.
Date (Phoenix dactylifera L.) seeds (PDL) have recently evoked significant attention for their therapeutic potential against numerous diseases. Aflatoxin B1 (AFB1) is an inevitable environmental hazard that pollutes foods and may harm the heart. This study investigated the beneficial effect of PDL against cardiac toxicity induced by AFB1 in male offspring. Female albino rats received PDL (200 mg/kg) orally for 14 days before mating till weaning and AFB1 (50 μg/kg) intramuscularly throughout gestation and lactation. At postnatal day 60, male offspring hearts were collected. Compared to AFB1 intoxicated group, PDL-treated offspring displayed improved cardiac biomarkers, an increase in their antioxidant defense, and a decrease in the cardiac proinflammatory cytokines. Additionally, a reduction in the expression levels of Bcl2 and Nrf2 was observed, with genes linked to increased cardiac caspase-3, Bax, ACE1, P53, and cytochrome C levels. In conclusion, PDL acts as a potential adjuvant agent for ameliorating cardiac toxicity and apoptosis resulting from exposure to AFB1. This is attributed to its antioxidative and anti-inflammatory effects, as well as its capacity to sequester free radicals within cardiac tissue.
海枣（Phoenix dactylifera L.）种子（PDL）最近因其对多种疾病的治疗潜力而引起了广泛关注。黄曲霉毒素B1（AFB1）是一种不可避免的环境危害，它会污染食物并可能损害心脏。本研究调查了PDL对AFB1诱导的雄性后代心脏毒性的有益作用。雌性白化大鼠在交配前至断奶期间口服PDL（200 mg/kg），持续14天，在整个妊娠期和哺乳期肌肉注射AFB1（50 μg/kg）。在出生后第60天，收集雄性后代的心脏。与AFB1中毒组相比，接受PDL治疗的后代心脏生物标志物得到改善，抗氧化防御能力增强，心脏促炎细胞因子减少。此外，观察到Bcl2和Nrf2的表达水平降低，同时与心脏中caspase-3、Bax、ACE1、P53和细胞色素C水平升高相关的基因也出现变化。总之，PDL可作为一种潜在的辅助剂，用于改善因接触AFB1而导致的心脏毒性和细胞凋亡。这归因于其抗氧化和抗炎作用，以及在心脏组织中螯合自由基的能力。
