McArdle H J, Douglas A J, Morgan E H
J Cell Physiol. 1985 Mar;122(3):405-9. doi: 10.1002/jcp.1041220310.
This paper describes a method for the culture of rat placental cells. The method involved separation of the basal layer from the labyrinth and sequential digestion of the cells. The cells were demonstrated not to be fibroblasts and are described in terms of their appearance under the light and electron microscopes. Transferrin and iron uptake by the cells was examined and compared with results achieved using other methods of study. The results showed that transferrin bound to receptors on the cell surface and that the transferrin, once bound, was taken into the cell. Only this internalized transferrin was capable of donating iron to the cells. The iron was accumulated within the cells and did not appear to be released to the incubation medium. The apparent dissociation constant (Ka) for transferrin was found to be 6.96 X 10(6) M-1, a value similar to that described by earlier workers. The placental cells had 3.4 X 10(11) binding sites/microgram DNA, equivalent to approximately 1 X 10(6) sites/cell. From these data, and from the rate of accumulation of iron by the cells, the receptor turnover time was estimated as being between 5 and 10 min.
本文描述了一种大鼠胎盘细胞的培养方法。该方法包括从迷路中分离基底层并对细胞进行顺序消化。这些细胞经证实不是成纤维细胞，并根据其在光学显微镜和电子显微镜下的外观进行了描述。检测了细胞对转铁蛋白和铁的摄取，并与使用其他研究方法获得的结果进行了比较。结果表明，转铁蛋白与细胞表面的受体结合，一旦结合，转铁蛋白就会被摄入细胞。只有这种内化的转铁蛋白能够向细胞提供铁。铁在细胞内积累，似乎不会释放到培养液中。转铁蛋白的表观解离常数（Ka）为6.96×10⁶ M⁻¹，这一数值与早期研究者描述的相似。胎盘细胞每微克DNA有3.4×10¹¹个结合位点，相当于每个细胞约1×10⁶个位点。根据这些数据以及细胞中铁的积累速率，估计受体周转时间在5到10分钟之间。
