Jaeger C B
J Comp Neurol. 1985 Jan 1;231(1):121-35. doi: 10.1002/cne.902310110.
Maturation of dopaminergic (DA) neurons and astroglia was studied in transplants of the substantia nigra grown for up to 7 months in the brain of rats. The investigation had three specific aims. The first was to observe effects of different transplant positions on the longevity of DA neurons. Second, the grafts were examined for changes of synaptic interactions and associations between DA neurons and astroglia. Third, an answer was sought to the question whether transplanted DA neurons migrate into the adjacent host brain. The grafts were taken from the ventral mesencephalon of rat embryos of different ages (day 14 to 18 of gestation) and placed into the cerebral cortex, tectum, cerebellum, or ventricles of newborn host animals. Following different times of survival the immunocytochemical localization of tyrosine hydroxylase (TH) and of glia filament protein (GFA) in the transplants were observed. In all of the transplantation sites, except for one, neurons of different morphologies that contained TH were found in the grafts. The cerebellar white matter of the host brain failed to support the long-term survival of DA neurons. The overall structure of mature substantia nigra grafts had some resemblance to intact substantia nigra (SN). On the ultrastructural level, it was found that morphological expression of some immature features of DA neurons, such as glial sheaths, somatic spines, and lack of oligodendroglia, persisted in mature grafts. Specific associations of DA neurons and astroglia in the grafts suggested that the cytoarchitectonic appearance of a given brain region may be related to the existence of particular neuron glia relationships. In contrast to intact SN, transplants revealed deficiencies in unlabeled pleomorphic boutons and contained some TH-immunoreactive terminals. Migration of DA neurons and their processes into the adjacent host brain was rarely observed.
在大鼠脑内生长长达7个月的黑质移植体中，对多巴胺能（DA）神经元和星形胶质细胞的成熟情况进行了研究。该研究有三个具体目标。第一个目标是观察不同移植位置对DA神经元寿命的影响。第二个目标是检查移植体中DA神经元与星形胶质细胞之间突触相互作用和联系的变化。第三个目标是寻找关于移植的DA神经元是否迁移到相邻宿主脑内这一问题的答案。移植体取自不同年龄（妊娠第14至18天）大鼠胚胎的腹侧中脑，并植入新生宿主动物的大脑皮质、顶盖、小脑或脑室。在不同存活时间后，观察移植体中酪氨酸羟化酶（TH）和胶质纤维酸性蛋白（GFA）的免疫细胞化学定位。在所有移植部位，除了一个部位外，在移植体中发现了含有TH的不同形态的神经元。宿主脑的小脑白质无法支持DA神经元的长期存活。成熟黑质移植体的整体结构与完整黑质（SN）有一些相似之处。在超微结构水平上，发现DA神经元一些不成熟特征的形态学表现，如胶质鞘、体细胞棘突和少突胶质细胞的缺乏，在成熟移植体中持续存在。移植体中DA神经元与星形胶质细胞的特定联系表明，特定脑区的细胞构筑外观可能与特定的神经元-胶质细胞关系的存在有关。与完整的SN相比，移植体显示出未标记的多形性终扣存在缺陷，并且含有一些TH免疫反应性终末。很少观察到DA神经元及其突起迁移到相邻宿主脑内。
