Zhang Juanxiang, Zha Xita, Yang Guowu, Ma Xiaoming, La Yongfu, Wu Xiaoyun, Guo Xian, Chu Min, Bao Pengjia, Yan Ping, Liang Chunnian
Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou 730050, China.
Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730050, China.
Animals (Basel). 2024 Dec 4;14(23):3506. doi: 10.3390/ani14233506.
The tyrosine protein kinase () gene, as a member of the non-receptor tyrosine kinase Tec family, plays a vital role in signal transduction mediation. Phospholipase C epsilon 1 (), a membrane-associated enzyme, is of paramount importance for the differentiation of myoblasts and the normal functioning of muscle tissue. In recent years, both of these genes have been reported to be associated with the economic traits of animals. This study aimed to investigate the relationship between single nucleotide polymorphisms (SNPs) in the and genes and growth traits in Ashidan yaks and to search for potential molecular marker loci that can influence Ashidan yak breeding. A cGPS liquid microarray was utilized to genotype 232 Ashidan yaks and to analyze correlations between two SNP loci in the and genes and yak body weight, body height, body length, and chest circumference at different periods. The results indicated that the g.55,999,531C>T locus of the gene and the g.342,350T>G locus of the gene were significantly correlated with the growth traits of Ashidan yaks. Among these, individuals with the CC genotype at the g.55,999,531C>T locus showed a significantly higher body length at 6 months old compared to TT individuals, and those with the CT genotype at 12 months old had a significantly higher chest circumference than TT individuals. At the g.342,350T>G locus, the body height of GG genotype individuals at 18 months of age was significantly higher than that of TT genotype individuals and TG genotype individuals. The above findings can be used as theoretical support for the subsequent improvement of Ashidan yak breeding.
酪氨酸蛋白激酶（）基因作为非受体酪氨酸激酶Tec家族的成员，在信号转导介导中起着至关重要的作用。磷脂酶Cε1（）是一种膜相关酶，对成肌细胞的分化和肌肉组织的正常功能至关重要。近年来，据报道这两个基因都与动物的经济性状有关。本研究旨在调查阿什旦牦牛和基因中的单核苷酸多态性（SNP）与生长性状之间的关系，并寻找可能影响阿什旦牦牛育种的潜在分子标记位点。利用cGPS液体微阵列对232头阿什旦牦牛进行基因分型，并分析和基因中两个SNP位点与不同时期牦牛体重、体高、体长和胸围之间的相关性。结果表明，基因的g.55,999,531C>T位点和基因的g.342,350T>G位点与阿什旦牦牛的生长性状显著相关。其中，g.55,999,531C>T位点CC基因型的个体在6月龄时的体长显著高于TT基因型个体，12月龄时CT基因型个体的胸围显著高于TT基因型个体。在g.342,350T>G位点，18月龄GG基因型个体的体高显著高于TT基因型个体和TG基因型个体。上述研究结果可为后续阿什旦牦牛育种改良提供理论支持。
