Kim Jieun, Eo Eun-Young, Kim Bokyong, Lee Heetak, Kim Jihoon, Koo Bon-Kyoung, Kim Hyung-Jun, Cho Sukki, Kim Jinho, Cho Young-Jae
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea.
Department of Biomedical Science, CHA University, Seongnam 13488, Republic of Korea.
Cells. 2024 Dec 2;13(23):1991. doi: 10.3390/cells13231991.
To develop in vitro respiratory models, it is crucial to identify the factors involved in epithelial cell differentiation. In this study, we comprehensively analyzed the effects of air-liquid interface (ALI) culture on epithelial cell differentiation using single-cell RNA sequencing (scRNA-seq). ALI culture induced a pronounced shift in cell composition, marked by a fivefold increase in ciliated cells and a reduction of more than half in basal cells. Transcriptional signatures associated with epithelial cell differentiation, analyzed using iPathwayGuide software, revealed the downregulation of and upregulation of as key signals for epithelial differentiation. Our findings highlight the efficacy of the ALI culture for replicating the human lung airway epithelium and provide valuable insights into the crucial factors that influence human ciliated cell differentiation.
为了建立体外呼吸模型，识别参与上皮细胞分化的因素至关重要。在本研究中，我们使用单细胞RNA测序（scRNA-seq）全面分析了气液界面（ALI）培养对上皮细胞分化的影响。ALI培养诱导了细胞组成的显著变化，其特征是纤毛细胞增加了五倍，基底细胞减少了一半以上。使用iPathwayGuide软件分析与上皮细胞分化相关的转录特征，发现[此处原文缺失相关基因名称]的下调和[此处原文缺失相关基因名称]的上调是上皮分化的关键信号。我们的研究结果突出了ALI培养在复制人肺气道上皮方面的功效，并为影响人纤毛细胞分化的关键因素提供了有价值的见解。
