Sun Yuxuan, Huang Fang, Chen Yan, Ning Nan, Hao Gang, Bi Xiufang
College of Pharmacy and Food, Southwest Minzu University, Chengdu 610041, China.
Foods. 2024 Nov 23;13(23):3756. doi: 10.3390/foods13233756.
This study aims to determine the effect of different high-pressure processing (HPP) conditions (100 MPa/300 MPa/500 MPa; 2 min/4 min/6 min) on copigmentation, specifically between chlorogenic acid (CA), epicatechin (Epi), gallic acid (GA), malvidin-3-O-galactoside (Mv-3-O-gal), and malvidin-3-O-arabinoside (Mv-3-O-ara), as well as the storage stability of the copigmentation solutions. The results showed that the influence of different HPP treatment conditions on copigmentation was not significant. HPP treatment did not significantly affect the λ, peak absorption, color parameters, and Mv-3-O-gal anthocyanin content when applied alone or in combination with CA and Epi. However, the color intensity and * value of Mv-3-O-gal with GA decreased by 3.2% ( < 0.05). The absorption peak, color, and content of Mv-3-O-ara were not affected by HPP alone or during copigmentation with CA, Epi, and GA. In addition, CA had the best effect on the co-coloring of Mv-3-O-gal, while GA was more successful in affecting Mv-3-O-ara during the storage period. Molecular dynamics simulations indicated that the aromatic ring of CA was closest to the A-C plane of Mv-3-O-gal (3.70 Å), resulting in a closer π-π stacking distance and higher bond energy. The favorable impact of GA on Mv-3-O-ara was because the A-C plane aromatic ring of Mv-3-O-ara and the aromatic D ring of GA formed "sandwich" stacking. The results indicated that combining HPP with polyphenols improved color and could be used to process raw materials containing malvidin, such as blueberries.
本研究旨在确定不同高压处理（HPP）条件（100兆帕/300兆帕/500兆帕；2分钟/4分钟/6分钟）对共色素化的影响，具体是对绿原酸（CA）、表儿茶素（Epi）、没食子酸（GA）、矢车菊素-3-O-半乳糖苷（Mv-3-O-）和矢车菊素-3-O-阿拉伯糖苷（Mv-3-O-ara）之间共色素化的影响，以及共色素化溶液的储存稳定性。结果表明，不同的HPP处理条件对共色素化的影响不显著。单独应用或与CA和Epi联合应用时，HPP处理对λ、峰值吸收、颜色参数和Mv-3-O-半乳糖苷花青素含量均无显著影响。然而，Mv-3-O-与GA组合时的颜色强度和*值下降了3.2%（<0.05）。单独的HPP处理或与CA、Epi和GA共色素化过程中，Mv-3-O-ara的吸收峰、颜色和含量均未受到影响。此外，CA对Mv-3-O-的共着色效果最佳，而GA在储存期对Mv-3-O-ara的影响更显著。分子动力学模拟表明，CA的芳香环最接近Mv-3-O-的A-C平面（3.70 Å），导致π-π堆积距离更近且键能更高。GA对Mv-3-O-ara的有利影响是因为Mv-3-O-ara的A-C平面芳香环与GA的芳香D环形成了“三明治”堆积。结果表明，将HPP与多酚结合可改善颜色，可用于加工含有矢车菊素的原料，如蓝莓。
