Yang Qianhui, Feng Zhiqiang, Chen Qian, Liu Haotian, Liu Qian, Sun Fangda, Kong Baohua
College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Foods. 2024 Nov 26;13(23):3808. doi: 10.3390/foods13233808.
Seventeen strains of LAB (lactic acid bacteria) were screened for their ability to form ZnPP (zinc protoporphyrin) by measuring fluorescence intensity. Three strains ( JX11, MDJ8, and Q) exhibited notable ZnPP-forming ability. The ferrochelatase enzyme activity of JX11 was significantly higher than that of the other two strains ( < 0.05). The three selected strains were then inoculated into minced meat to observe their effect on color development and quality properties. The *-values of the bacteria-inoculated groups were significantly higher than those of the control group and lower than those of the nitrite group ( < 0.05). The visible bright red color of the inoculated groups was stronger than that of the control and inferior to the nitrite group, especially in cooked minced meat. The fluorescence intensities in inoculated groups were significantly higher than those of the control and nitrite groups ( < 0.05). The UV-Vis absorbance data at 417 nm indicated that inoculated groups exhibited higher absorbance compared to the control group ( < 0.05). These results indicate that high ZnPP-forming bacteria can enhance the color of meat products and these have certain potential to replace nitrite in meat products.
通过测量荧光强度,筛选出17株乳酸菌(LAB)形成锌原卟啉(ZnPP)的能力。三株菌株(JX11、MDJ8和Q)表现出显著的ZnPP形成能力。JX11的亚铁螯合酶活性显著高于其他两株菌株(P<0.05)。然后将这三株筛选出的菌株接种到碎肉中,观察它们对颜色形成和品质特性的影响。接种细菌组的a*值显著高于对照组,低于亚硝酸盐组(P<0.05)。接种组可见的亮红色比对照组更强,比亚硝酸组差,尤其是在熟碎肉中。接种组的荧光强度显著高于对照组和亚硝酸盐组(P<0.05)。417nm处的紫外可见吸收数据表明,接种组的吸光度高于对照组(P<0.05)。这些结果表明,高ZnPP形成细菌可以增强肉制品的颜色,并且在肉制品中具有一定替代亚硝酸盐的潜力。