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精油对(法布里丘斯)的化学成分、杀幼虫和杀卵活性以及酶抑制能力

Chemical Composition, Larvicidal and Ovicidal Activities, and Enzyme Inhibition Capacity of Essential Oils Against (Fabricius).

作者信息

Wang Lijun, Jing Siluo, Wang Shuang, Xing Zhikai, Qu Jiangyong, Wang Xumin

机构信息

College of Life Science, Yantai University, Yantai 264005, China.

出版信息

Plants (Basel). 2024 Nov 26;13(23):3315. doi: 10.3390/plants13233315.

Abstract

Due to their effectiveness at low doses and relative safety for non-target species, plant essential oils (EOs) are considered ideal alternatives to conventional pesticides for pest control. In this study, the chemical composition of () EO was construed by Gas Chromatography-Mass Spectrometry (GC-MS), and its larvicidal and ovicidal activity against omnivorous pests () was assessed. The effects of EO on the activities of antioxidant detoxification enzymes were also measured. GC-MS analysis revealed that the main constituents of EO were thymol (42.1%), p-cymene (22.4%), and γ-terpinene (18.6%). In the larvicidal toxicity experiment, the EO demonstrated LC50 values of 0.606 and 0.664 mg/mL against the second- and third-instar larvae of respectively, after 48 h exposure. Moreover, an EC value of 0.905 mg/mL was measured against eggs. In , EO treatment reduced the enzymatic activity of ESTs and GST and, conversely, increased the enzymatic activity of AChE. Overall, this study demonstrated that EO has the potential to be implemented as a novel eco-friendly insecticide against .

摘要

由于植物精油(EOs)在低剂量下具有有效性且对非目标物种相对安全,因此被认为是用于害虫防治的传统杀虫剂的理想替代品。在本研究中,通过气相色谱 - 质谱联用仪(GC - MS)分析了()EO的化学成分,并评估了其对杂食性害虫()的杀幼虫和杀卵活性。还测定了EO对抗氧化解毒酶活性的影响。GC - MS分析表明,EO的主要成分是百里香酚(42.1%)、对伞花烃(22.4%)和γ - 萜品烯(18.6%)。在杀幼虫毒性实验中,暴露48小时后,EO对()的二龄和三龄幼虫的LC50值分别为0.606和0.664 mg/mL。此外,测得对()卵的EC值为0.905 mg/mL。在()中,EO处理降低了酯酶(ESTs)和谷胱甘肽 - S - 转移酶(GST)的酶活性,相反,增加了乙酰胆碱酯酶(AChE)的酶活性。总体而言,本研究表明EO有潜力作为一种新型环保杀虫剂用于防治()。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1562/11644745/0990deb3c333/plants-13-03315-g001.jpg

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