Puzanskiy Roman K, Kirpichnikova Anastasia A, Bogdanova Ekaterina M, Prokopiev Ilya A, Shavarda Alexey L, Romanyuk Daria A, Vanisov Sergey A, Yemelyanov Vladislav V, Shishova Maria F
Laboratory of Analytical Phytochemistry, Komarov Botanical Institute of the Russian Academy of Sciences, 197022 St. Petersburg, Russia.
Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia.
Plants (Basel). 2024 Dec 6;13(23):3426. doi: 10.3390/plants13233426.
Tobacco BY-2 cell culture is one of the most widely used models in plant biology. The main advantage of BY-2 suspension cultures is the synchronization of cell development and the appearance of polar elongation. In batch culture, BY-2 cells passed through the lag, proliferation, elongation, and stationary phases. During this process, the composition of the growth medium changed dramatically. Sucrose was rapidly eliminated; hexose first accumulated and then depleted. The medium's pH initially decreased and then rose with aging. As a result of the crosstalk between the internal and external stimuli, cells pass through complicated systemic rearrangements, which cause metabolomic alterations. The early stages were characterized by high levels of amino acids and sterols, which could be interpreted as the result of synthetic activity. The most intense rearrangements occurred between the proliferation and active elongation stages, including repression of amino acid accumulation and up-regulation of sugar metabolism. Later stages were distinguished by higher levels of secondary metabolites, which may be a non-specific response to deteriorating conditions. Senescence was followed by some increase in fatty acids and sterols as well as amino acids, and probably led to self-destructive processes. A correlation analysis revealed relationships between metabolites' covariation, their biochemical ratio, and the growth phase.
烟草BY-2细胞培养是植物生物学中应用最广泛的模型之一。BY-2悬浮培养的主要优点是细胞发育同步化以及出现极性伸长。在分批培养中，BY-2细胞经历延迟期、增殖期、伸长期和静止期。在此过程中，生长培养基的成分发生了巨大变化。蔗糖迅速消耗；己糖先积累后耗尽。培养基的pH值最初下降，然后随着老化而上升。由于内外刺激之间的相互作用，细胞经历复杂的系统重排，导致代谢组学改变。早期的特征是氨基酸和甾醇水平较高，这可以解释为合成活性的结果。最剧烈的重排发生在增殖期和活跃伸长期之间，包括氨基酸积累的抑制和糖代谢的上调。后期的特征是次生代谢物水平较高，这可能是对恶化条件的非特异性反应。衰老之后，脂肪酸、甾醇以及氨基酸有所增加，可能导致自我破坏过程。相关性分析揭示了代谢物协变、它们的生化比率与生长阶段之间的关系。
