用卟啉脂蛋白光动力疗法诱导应力纤维形成的可视化

Visualization of Stress Fiber Formation Induced by Photodynamic Therapy with Porphylipoprotein.

作者信息

Taninaka Atsushi, Kurokawa Hiromi, Kamiyanagi Mayuka, Takeuchi Osamu, Matsui Hirofumi, Shigekawa Hidemi

机构信息

Institute of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8573, Japan.

TAKANO Co., Ltd., Miyada-mura, Kamiina-gun, Nagano 399-4301, Japan.

出版信息

Nanomaterials (Basel). 2024 Nov 21;14(23):1862. doi: 10.3390/nano14231862.

DOI:10.3390/nano14231862

PMID:39683251

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11643885/

Abstract

We investigated stress fiber formation induced by photodynamic therapy (PDT) with porphylipoprotein (PLP) by observing actin filaments by super-resolution confocal microscopy and measuring the cellular elastic modulus by atomic force microscopy. We identified different intracellular mechanisms of stress fiber formation between RGM1 epithelial cells, which were derived from rat gastric mucosa, and RGK1 cells, which were cancer-like mutants of RGM1. Our findings show that when PLP is used as a photosensitizer in PDT, it selectively induces necrosis in tumors with minimal impact on the surrounding normal tissues, as it is less likely to cause blood flow obstruction.

摘要

我们通过超分辨率共聚焦显微镜观察肌动蛋白丝，并通过原子力显微镜测量细胞弹性模量，研究了卟啉脂蛋白（PLP）介导的光动力疗法（PDT）诱导的应力纤维形成。我们确定了源自大鼠胃黏膜的RGM1上皮细胞和RGM1的癌样突变体RGK1细胞之间应力纤维形成的不同细胞内机制。我们的研究结果表明，当PLP用作PDT中的光敏剂时，它能选择性地诱导肿瘤坏死，对周围正常组织的影响最小，因为它不太可能导致血流阻塞。