Park Jongbeom, Park Sujeong, Evelina Grace, Kim Sunghee, Jin Yong-Su, Chi Won-Jae, Kim In Jung, Kim Soo Rin
School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
Research Institute of Tailored Food Technology, Kyungpook National University, Daegu 41566, Republic of Korea.
Molecules. 2024 Dec 2;29(23):5695. doi: 10.3390/molecules29235695.
Cellulosic biomass hydrolysates are rich in glucose and xylose, but most microorganisms, including , are unable to utilize xylose effectively. To address this limitation, we engineered a strain optimized for xylose metabolism through the xylose oxidoreductase pathway and promoter optimization. A promoter library with varying strengths was used to fine-tune the expression levels of the , , and genes, resulting in a strain with a strong promoter for and weaker promoters for and . This engineered strain exhibited superior growth, achieving 14 g cells/L and a maximal growth rate of 0.4 g cells/L-h in kenaf hydrolysate, outperforming a native strain by 17%. This study is the first to report the introduction of the xylose oxidoreductase pathway into , demonstrating its potential as an industrial platform for producing yeast protein and other products from cellulosic biomass.
纤维素生物质水解产物富含葡萄糖和木糖，但大多数微生物，包括……，都无法有效利用木糖。为解决这一限制，我们通过木糖氧化还原酶途径和启动子优化构建了一种针对木糖代谢进行优化的……菌株。使用具有不同强度的启动子文库来微调……、……和……基因的表达水平，从而得到一种菌株，其……具有强启动子，而……和……具有较弱启动子。这种工程菌株表现出优异的生长性能，在红麻水解产物中细胞密度达到14 g/L，最大生长速率为0.4 g细胞/（L·h），比天然菌株性能高出17%。本研究首次报道了将木糖氧化还原酶途径引入……，证明了其作为从纤维素生物质生产酵母蛋白和其他产品的工业平台的潜力。
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