毕赤酵母中增强的甲醇-木糖共利用策略

Enhanced methanol-xylose co-utilization strategy in Komagataella phaffii.

作者信息

Li Kang, Yang Shaojie, Wang Tengfei, Zhan Chunjun, Bai Zhonghu, Yang Yankun

机构信息

School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.

State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Shandong Academy of Sciences, Qilu University of Technology, Jinan, Shandong, China; Key Laboratory of Shandong Microbial Engineering, School of Bioengineering, Shandong Academy of Sciences, Qilu University of Technology, Jinan, Shandong, China.

出版信息

J Biotechnol. 2025 Mar;399:117-126. doi: 10.1016/j.jbiotec.2025.01.017. Epub 2025 Jan 26.

DOI:10.1016/j.jbiotec.2025.01.017

PMID:39875025

Abstract

Bio-manufacturing based on non-food carbon sources is conducive to alleviating the global food crisis and greenhouse effect. However, the mechanism of the utilization of methanol and xylose in Komagataella phaffii based on endogenous metabolic pathways has not been fully explored. In this study, transcriptomics revealed a positive correlation between methanol metabolic efficiency and the transcription level of genes related to xylose metabolism and phosphate metabolism. By providing sufficient phosphate to the strain, the methanol utilization rate of the Komagataella phaffii GA01 strain was improved, and the final biomass reached 7.5 g DCW/L. Metabolomics further confirmed that methanol could effectively activate xylose metabolism of the strain, and the consumption rates of methanol and xylose of the Komagataella phaffii GA01 strain could reach 3.87 g/L/d and 1.83 g/L/d, which were 34 % and 357.5 % higher than that of the wild-type strain, respectively. This study further promotes the application of methanol and xylose in microbial fermentation.

摘要

基于非粮食碳源的生物制造有利于缓解全球粮食危机和温室效应。然而，基于内源代谢途径的毕赤酵母对甲醇和木糖的利用机制尚未得到充分探索。在本研究中，转录组学揭示了甲醇代谢效率与木糖代谢和磷酸盐代谢相关基因转录水平之间存在正相关。通过向菌株提供充足的磷酸盐，提高了毕赤酵母GA01菌株的甲醇利用率，最终生物量达到7.5 g DCW/L。代谢组学进一步证实，甲醇可有效激活该菌株的木糖代谢，毕赤酵母GA01菌株的甲醇和木糖消耗速率分别可达3.87 g/L/d和1.83 g/L/d，分别比野生型菌株高34%和357.5%。本研究进一步推动了甲醇和木糖在微生物发酵中的应用。