小麦（Triticum aestivum L.）中油菜素唑抗性（Brassinazole-Resistant，BZR）基因家族的鉴定以及TaBZR1的分子克隆与功能表征

Identification of the Brassinazole-Resistant () Gene Family in Wheat ( L.) and the Molecular Cloning and Functional Characterization of .

作者信息

Zhang Yan, Qin Jingzi, Hou Jinna, Liu Congcong, Geng Shenghui, Qin Maomao, Li Wenxu, Dai Ziju, Wu Zhengqing, Lei Zhensheng, Zhou Zhengfu

机构信息

Henan Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.

National Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.

出版信息

Int J Mol Sci. 2024 Nov 22;25(23):12545. doi: 10.3390/ijms252312545.

DOI:10.3390/ijms252312545

PMID:39684257

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11641346/

Abstract

Brassinazole-resistant (BZR) transcription factors are important transcription factors in Brassinosteroid (BR)-responsive gene expression. However, limited knowledge exists regarding the genes in wheat and a limited number of family genes have been previously reported in wheat. In this study, the synteny analyses of the genes suggested that gene duplication events have played an essential role in the family during evolution. The results of RT-qPCR and transcriptome data analyses exhibited remarkable expression patterns in the genes in different tissues and under different treatments. The yeast two-hybrid (Y2H) screen result showed that the TaBZR2.1 protein interacts with Argonaute 4 (AGO4). Taken together, our results not only provide us a basis for understanding the molecular characteristics and expression patterns of the family genes but also offered the functional characterization of in wheat.

摘要

油菜素唑抗性（BZR）转录因子是油菜素类固醇（BR）响应基因表达中的重要转录因子。然而，关于小麦中的这些基因的了解有限，之前在小麦中仅报道了少数该家族基因。在本研究中，这些基因的共线性分析表明基因复制事件在进化过程中对该家族起到了重要作用。RT-qPCR和转录组数据分析结果显示这些基因在不同组织和不同处理下呈现出显著的表达模式。酵母双杂交（Y2H）筛选结果表明TaBZR2.1蛋白与AGO4相互作用。综上所述，我们的结果不仅为理解该家族基因的分子特征和表达模式提供了基础，还对小麦中该基因的功能特性进行了研究。