六种苹果砧木耐盐性评价

Evaluation of Salt Resistance of Six Apple Rootstocks.

作者信息

Li Lun, Chen Guolin, Sun Qingrong, Wang Qing, Wang Sen, Wang Haibo, Ni Zhihua, Jiang Caina, Li Linguang, Li Tianhong

机构信息

Frontiers Science Center for Molecular Design Breeding, College of Horticulture, China Agricultural University, Beijing 100193, China.

Shandong Institute of Pomology, Taian 271000, China.

出版信息

Int J Mol Sci. 2024 Nov 22;25(23):12568. doi: 10.3390/ijms252312568.

DOI:10.3390/ijms252312568

PMID:39684281

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11640993/

Abstract

Apples ( are important fruits in China; however, salt stress is severe in northern regions, and the key to plant resistance to salt stress lies in the rootstock. Therefore, it is necessary to explore rootstocks with strong salt resistance for the development of the apple industry. This study used tissue culture seedlings of six apple rootstocks, namely, '71-3-150', '54-118', 'M9T337', 'GM256', 'ML176', and 'ML2', as experimental materials. The seedlings were treated with a medium containing 150 mM NaCl, and the physiological indicators and related gene expression responses of several rootstocks were studied after salt stress. The results showed that salt stress affects the growth of both the aboveground and underground parts of plants. Through physiological indicators and the related gene expression responses of rootstocks, it was observed that salt stress significantly increased Na contents in different rootstocks. Simultaneously, the activity of various antioxidant enzymes and the expression levels of related genes also increased. In summary, by analyzing the parameters of various physiological indicators, it can be concluded that among the studied rootstocks, the '71-3-150' and '54-118' rootstocks have the strongest resistance to salt stress, while the 'M9T337' and 'GM256' rootstocks exhibit moderate resistance, and the 'ML176' and 'ML2' rootstocks have the weakest resistance.

摘要

苹果是中国重要的水果；然而，北方地区盐胁迫严重，而植物抗盐胁迫的关键在于砧木。因此，为了苹果产业的发展，有必要探索耐盐性强的砧木。本研究以6种苹果砧木的组培苗，即‘71 - 3 - 150’、‘54 - 118’、‘M9T337’、‘GM256’、‘ML176’和‘ML2’为试验材料。将幼苗用含150 mM NaCl的培养基处理，研究盐胁迫后几种砧木的生理指标及相关基因表达响应。结果表明，盐胁迫影响植物地上和地下部分的生长。通过砧木的生理指标及相关基因表达响应观察到，盐胁迫显著增加了不同砧木中的Na含量。同时，各种抗氧化酶的活性及相关基因的表达水平也有所增加。综上所述，通过分析各项生理指标参数可以得出，在所研究的砧木中，‘71 - 3 - 150’和‘54 - 118’砧木对盐胁迫的抗性最强，而‘M9T337’和‘GM256’砧木表现出中等抗性，‘ML176’和‘ML2’砧木的抗性最弱。