Zhang Bi-Tian, Leung Ping-Chung, Wong Chun-Kwok, Wang Dong-Jie
Institute of Chinese Medicine, State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Hong Kong, China.
Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, China.
Int J Mol Sci. 2024 Dec 2;25(23):12952. doi: 10.3390/ijms252312952.
Glioma is a highly invasive brain cancer that is difficult to treat due to its complex molecular characteristics and poor prognosis. The COVID-19 pandemic has introduced additional clinical challenges for cancer patients, especially those with glioma. This study explored the molecular interactions between glioma and COVID-19 using integrated bioinformatics methods, including enrichment analysis, survival analysis, and molecular docking, focusing on the PI3K-Akt signaling pathway and the immunomodulatory role of vitamin D. From gene expression data of glioma and COVID-19, 203 common differentially expressed genes were identified, and six prognostic key genes-MYBL2, RBM6, VEPH1, AHNAK2, GNG10, and DUSP14-were further determined. After intersecting with vitamin D targets five prognostic key genes were determined-MYBL2, RBM6, VEPH1, AHNAK2 and GNG10. These genes play significant roles in the PI3K-Akt pathway and potentially interact with vitamin D. Molecular docking and single-cell RNA sequencing analyses suggest that vitamin D may improve the prognosis of glioma patients infected with COVID-19 by regulating these key genes and the PI3K-Akt pathway. The findings reveal molecular links between glioma and COVID-19, thereby providing new insights for developing targeted therapeutic strategies.
神经胶质瘤是一种具有高度侵袭性的脑癌，由于其复杂的分子特征和较差的预后，难以治疗。新冠疫情给癌症患者，尤其是神经胶质瘤患者带来了额外的临床挑战。本研究使用综合生物信息学方法，包括富集分析、生存分析和分子对接，探索神经胶质瘤与新冠病毒之间的分子相互作用，重点关注PI3K-Akt信号通路以及维生素D的免疫调节作用。从神经胶质瘤和新冠病毒的基因表达数据中，确定了203个共同差异表达基因，并进一步确定了6个预后关键基因——MYBL2、RBM6、VEPH1、AHNAK2、GNG10和DUSP14。与维生素D靶点交叉后，确定了5个预后关键基因——MYBL2、RBM6、VEPH1、AHNAK2和GNG10。这些基因在PI3K-Akt通路中发挥重要作用，并可能与维生素D相互作用。分子对接和单细胞RNA测序分析表明，维生素D可能通过调节这些关键基因和PI3K-Akt通路来改善感染新冠病毒的神经胶质瘤患者的预后。这些发现揭示了神经胶质瘤与新冠病毒之间的分子联系，从而为制定靶向治疗策略提供了新的见解。
