Zhang Geng, Du Yuxuan, Wang Xinying, Zhang Yuge, Zhang Shili, Li Mingyang, Li Xiaojuan, Zhang Guifang
State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2024 Dec 7;25(23):13156. doi: 10.3390/ijms252313156.
root regeneration is the process by which adventitious roots form around the wound site from wounded or detached plant organs. The root regeneration process has been widely exploited in cutting technology used for vegetative propagation. Here, we employed detached leaf explants from to form adventitious roots for studying the process of root regeneration. GUS staining showed that the expression of () was gradually increased surrounding the wound site of leaf explants during adventitious root formation. Semi-thin sections further showed that the expression pattern of was closely linked to the formation of adventitious roots. Next, genetic analyses confirmed that the gene was involved in root regeneration. Furthermore, RNA sequencing (RNA-seq) of the leaf explants revealed that stress-related genes might be involved in -mediated adventitious root formation. Specifically, genes associated with the hydrogen peroxide catabolic process and oxidative stress response were predominantly upregulated in the mutant. In contrast, genes involved in the response to salicylic acid were largely downregulated in the mutant. Overall, our study indicates that the mutation in might upregulate the expression of genes involved in reactive oxygen species metabolism or suppress the expression of genes associated with salicylic acid synthesis, thus promoting the formation of adventitious roots. These findings suggest that is a potential candidate for the genetic improvement of adventitious rooting in cuttings.
根再生是指不定根从受伤或分离的植物器官在伤口部位周围形成的过程。根再生过程已在用于营养繁殖的扦插技术中得到广泛应用。在此，我们利用离体叶片外植体形成不定根来研究根再生过程。GUS染色显示，在不定根形成过程中，（）在叶片外植体伤口部位周围的表达逐渐增加。半薄切片进一步表明，的表达模式与不定根的形成密切相关。接下来，遗传分析证实该基因参与根再生。此外，对叶片外植体的RNA测序（RNA-seq）表明，与胁迫相关的基因可能参与介导的不定根形成。具体而言，与过氧化氢分解代谢过程和氧化应激反应相关的基因在突变体中主要上调。相反，参与水杨酸反应的基因在突变体中大多下调。总体而言，我们的研究表明，中的突变可能上调参与活性氧代谢的基因表达或抑制与水杨酸合成相关的基因表达，从而促进不定根的形成。这些发现表明，是扦插中不定根遗传改良的潜在候选基因。
