Wang Shiwen, Liu Min, Hu Dongping, Dong Zhicheng, Zhao Zhong
Ministry of Education Key Laboratory for Cellular Dynamics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Guangzhou Key Laboratory of Crop Gene Editing, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, China.
Nat Chem Biol. 2025 Apr;21(4):567-576. doi: 10.1038/s41589-024-01737-8. Epub 2024 Sep 12.
Superoxide anion is thought to be a natural by-product with strong oxidizing ability in all living organisms and was recently found to accumulate in plant meristems to maintain stem cells in the shoot and undifferentiated meristematic cells in the root. Here we show that the DNA demethylase repressor of silencing 1 (ROS1) is one of the direct targets of superoxide in stem cells. The Fe-S clusters in ROS1 are oxidized by superoxide to activate its DNA glycosylase/lyase activity. We demonstrate that superoxide extensively participates in the establishment of active DNA demethylation in the Arabidopsis genome and that ARABIDOPSIS RESPONSE REGULATOR 12 acts downstream of ROS1-mediated superoxide signaling to maintain stem cell fate. Our results provide a mechanistic framework for superoxide control of the stem cell niche and demonstrate how redox and DNA demethylation interact to define stem cell fate in plants.
超氧阴离子被认为是所有生物中具有强氧化能力的天然副产物，最近发现它在植物分生组织中积累，以维持茎尖的干细胞和根中未分化的分生细胞。在这里，我们表明沉默1的DNA去甲基化酶阻遏物（ROS1）是干细胞中超氧的直接靶点之一。ROS1中的铁硫簇被超氧氧化，以激活其DNA糖基化酶/裂解酶活性。我们证明超氧广泛参与拟南芥基因组中活性DNA去甲基化的建立，并且拟南芥响应调节因子12在ROS1介导的超氧信号下游起作用，以维持干细胞命运。我们的结果为超氧对干细胞微环境的控制提供了一个机制框架，并证明了氧化还原和DNA去甲基化如何相互作用来定义植物中的干细胞命运。
