Parenrengi Andi, Suryati Emma, Syah Rachman, Tenriulo Andi, Lante Samuel, Zainuddin Elmi Nurhaidah, Aliah Ratu Siti, Farizah Nuril, Nawang Agus, Sulaeman Sulaeman, Makmur Makmur, Rosmiati Rosmiati, Gunarto Gunarto, Herlinah Herlinah
Research Center for Fishery, National Research and Innovation Agency, Cibinong, Indonesia.
Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar, Indonesia.
PeerJ. 2024 Dec 13;12:e18670. doi: 10.7717/peerj.18670. eCollection 2024.
The banana plant is claimed to contain a serotonin compound that has the potential to stimulate and improve the reproductive performance of crustacean species. This study aimed to isolate and characterize the serotonin compound from the banana hump and its application to enhance the reproductive performance of tiger shrimp broodstock.
Banana hump as a part of the plant was extracted by using the maceration technique. The chemical structure of the serotonin compound was identified and characterized based on spectroscopic data, including high-performance liquid chromatography (HPLC), ultraviolet-visible (UV-Vis) spectroscopy, and infrared (IR) spectroscopy, and a comparison was made with the standard compound and the literature. The extract herb in a dose of 50 µg/g body weight was injected into the tiger shrimp broodstock in four-time administrations. During 2 months of broodstock gonadal maturation, the parameters of molting, reproduction, and gene expression related to reproduction were observed.
Based on the chemical structure analysis, the stimulant component of the banana hump was identified as a serotonin compound (5-hydroxytryptamine) at a concentration of 0.7% of dry weight. The number of spawned broodstock was higher in the serotonin extract treatment (60%) than in the control treatment (40%), and the broodstock injected serotonin spawned up to the second re-maturation. In contrast, no re-maturation was obtained in the control treatment. The egg number was significantly higher using the serotonin extract (286,550 ± 46,402 eggs) than the control shrimp (148,585 ± 23,647 eggs), in which the serotonin extract treatment showed a comparatively larger egg diameter number. The higher expression of the genes related to female and male reproduction was observed in the tiger shrimp injected with serotonin extract than in the control treatment.
据称香蕉植株含有一种血清素化合物，有可能刺激并改善甲壳类物种的繁殖性能。本研究旨在从香蕉果柄中分离并鉴定血清素化合物，并将其应用于提高虎虾亲虾的繁殖性能。
采用浸渍技术提取香蕉植株的果柄部分。基于光谱数据，包括高效液相色谱（HPLC）、紫外可见（UV-Vis）光谱和红外（IR）光谱，对血清素化合物的化学结构进行鉴定和表征，并与标准化合物及文献进行比较。将剂量为50μg/g体重的提取物草药分四次注射到虎虾亲虾体内。在亲虾性腺成熟的2个月期间，观察蜕皮、繁殖以及与繁殖相关的基因表达等参数。
基于化学结构分析，香蕉果柄的刺激成分被鉴定为一种血清素化合物（5-羟色胺），其干重浓度为0.7%。血清素提取物处理组中产卵亲虾的数量（60%）高于对照组（40%），注射血清素的亲虾产卵直至第二次重新成熟。相比之下，对照组未出现重新成熟的情况。使用血清素提取物时的卵数（286,550±46,402个卵）显著高于对照虾（148,585±23,647个卵），其中血清素提取物处理组的卵直径相对较大。与对照组相比，注射血清素提取物的虎虾中观察到与雌雄繁殖相关基因的更高表达。
