血清外泌体长链非编码RNA DLEU1在宫颈癌患者中的表达及临床意义

The expression and clinical significance of serum exosomal-long non-coding RNA DLEU1 in patients with cervical cancer.

作者信息

Chen Yu, Cui Facai, Wu Xiaoyu, Zhao Weifeng, Xia Qingxin

机构信息

Department of Pathology, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, PR China.

Department of Clinical Laboratory, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, PR China.

出版信息

Ann Med. 2025 Dec;57(1):2442537. doi: 10.1080/07853890.2024.2442537. Epub 2024 Dec 17.

DOI:10.1080/07853890.2024.2442537

PMID:39687982

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11654034/

Abstract

BACKGROUND

Accumulating evidence has demonstrated that the long non-coding RNA (lncRNA) lymphocytic leukaemia deletion gene 1 (DLEU1) is abnormally overexpressed in many cancer types, including cervical cancer (CC). However, the potential clinical significance of DLEU1 in serum exosomes of patients with CC remains unclear.

METHODS

The expression of serum exosomal DLEU1 was detected by quantitative real-time polymerase chain reaction (qRT-PCR). A receiver operating characteristic (ROC) curve was plotted to evaluate the clinical diagnostic efficacy of DLEU1. The Kaplan-Meier survival curve and Cox proportional hazards model were used to assess the effect of DLEU1 on postoperative recurrence, metastasis and prognosis among patients with CC.

RESULTS

Our research showed that DLEU1 expression in the serum exosomes of patients with CC was significantly upregulated compared to that in patients with cervical intraepithelial neoplasia (CIN) and healthy controls (HCs) (both < .001). DLEU1 relative expression was significantly correlated with tumour size, cervical invasion depth, pathological grade, International Federation of Gynecology and Obstetrics (FIGO) stage and lymph node metastasis among patients with CC ( < .01 all). The combined detection of DLEU1, carbohydrate antigen 125 (CA-125) and squamous cell carcinoma (SCC) exhibited significantly higher diagnostic efficiency ( < .01). Furthermore, the overall survival (OS) and disease-free survival (DFS) of CC patients in the high DLEU1 expression group were markedly lower than those in the low DLEU1 expression group (both < .01). Cox univariate and multivariate regression analyses indicated that DLEU1 was an independent risk factor for postoperative recurrence and metastasis in CC patients.

CONCLUSIONS

Our findings suggest that serum exosome DLEU1 has certain clinical value for diagnosing, monitoring recurrence and metastasis, and evaluating CC prognosis.

摘要

背景

越来越多的证据表明，长链非编码RNA（lncRNA）淋巴细胞白血病缺失基因1（DLEU1）在包括宫颈癌（CC）在内的多种癌症类型中异常高表达。然而，DLEU1在CC患者血清外泌体中的潜在临床意义仍不清楚。

方法

采用定量实时聚合酶链反应（qRT-PCR）检测血清外泌体DLEU1的表达。绘制受试者工作特征（ROC）曲线以评估DLEU1的临床诊断效能。采用Kaplan-Meier生存曲线和Cox比例风险模型评估DLEU1对CC患者术后复发、转移及预后的影响。

结果

我们的研究表明，与宫颈上皮内瘤变（CIN）患者和健康对照（HCs）相比，CC患者血清外泌体中DLEU1的表达显著上调（均P<0.001）。CC患者中DLEU1相对表达与肿瘤大小、宫颈浸润深度、病理分级、国际妇产科联盟（FIGO）分期及淋巴结转移显著相关（均P<0.01）。DLEU1、糖类抗原125（CA-125）和鳞状细胞癌（SCC）联合检测显示出显著更高的诊断效率（P<0.01）。此外，高DLEU1表达组CC患者的总生存期（OS）和无病生存期（DFS）明显低于低DLEU1表达组（均P<0.01）。Cox单因素和多因素回归分析表明，DLEU1是CC患者术后复发和转移的独立危险因素。