心肌活检钻：构造细节与技术

Myocardial biopsy drill: construction details and techniques.

作者信息

Breyer R H, Meredith J W, Kon N D, Mills S A

出版信息

J Surg Res. 1985 Feb;38(2):134-7. doi: 10.1016/0022-4804(85)90018-6.

Abstract

The modification of currently available dental equipment to construct a myocardial biopsy drill by the technique of Poole et al. (P. E. Poole, G. F. Norris, R. M. Lewis, and J. W. Covell, J. Appl. Physiol. 24: 832, 1968) is described. In dogs, five to seven full-thickness myocardial cores 3 mm in diameter and weighing 40 to 70 mg could be obtained consistently with the drill without affecting left ventricular function. The positive pressure/negative pressure system allowed separation of contaminating blood from the myocardial sample and rapid myocardial freezing. Assessment by a modification of the technique of Estabrook et al. (R. W. Estabrook, J. R. Williamson, R. Frenkel, and P. K. Maitra, In R. W. Estabrook and M. E. Pullman (Eds.), Methods in Enzymology, New York: Academic Press, 1967. Vol. 10, p. 474). yielded myocardial ATP levels of 32.12 +/- 0.90 nmole/mg of protein.

摘要

本文描述了通过Poole等人（P.E. Poole、G.F. Norris、R.M. Lewis和J.W. Covell，《应用生理学杂志》24: 832，1968年）的技术对现有牙科设备进行改造，以构建心肌活检钻。在犬类中，使用该活检钻能够持续获取五到七个直径3毫米、重量40至70毫克的全层心肌组织芯，且不会影响左心室功能。正压/负压系统可将污染血液与心肌样本分离，并实现心肌快速冷冻。采用Estabrook等人（R.W. Estabrook、J.R. Williamson、R.Frenkel和P.K. Maitra，载于R.W. Estabrook和M.E. Pullman编著的《酶学方法》，纽约：学术出版社，1967年。第10卷，第474页）技术的一种改良方法进行评估，得出心肌ATP水平为32.12±0.90纳摩尔/毫克蛋白质。