Li Yong, Wang Lan-Lan, Feng Li-Li, Duan De-Yong, Mihaljica Darko, Cheng Tian-Yin
Research Center for Parasites & Vectors, College of Veterinary Medicine, Hunan Agricultural University, Hunan Province 410128, Changsha, China.
Group for Medical Entomology, Centre of Excellence for Food- and Vector-Borne Zoonoses, Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia.
Parasitol Res. 2024 Dec 17;123(12):409. doi: 10.1007/s00436-024-08422-8.
In this study, the cDNAs of heat shock cognate 90 protein 1 (HfHspc1) and heat shock cognate 90 protein 4 (HfHspc4) from Haemaphysalis flava (Acari: Ixodidae) were obtained using the rapid amplification of cDNA ends (RACE) approach, and the expression patterns of HfHspc1 and HfHspc4 in different developmental stages, engorgement stages and tick organs were analyzed by qPCR. The full length of HfHspc1 was 2411 bp, and its open reading frame (ORF) was 2196 bp, encoding a protein of 732 aa, containing five HSPC family signatures, with MEEVD motif at its extreme C-terminal. The full length of HfHspc4 was 2800 bp, and its ORF was 2364 bp, encoding a protein of 789 aa, containing a signal peptide and five family signatures, with HEEL motif at its extreme C-terminal. The expression of HfHspc1 and HfHspc4 was the highest in males, while it was significantly the highest in the ovaries of fully engorged females, potentially implying the roles of these proteins in the successful digestion of blood and development of eggs.
在本研究中，采用cDNA末端快速扩增（RACE）方法获得了黄褐血蜱（蜱螨亚纲：硬蜱科）的热休克同源蛋白1（HfHspc1）和热休克同源蛋白4（HfHspc4）的cDNA，并通过qPCR分析了HfHspc1和HfHspc4在不同发育阶段、饱血阶段和蜱器官中的表达模式。HfHspc1全长2411 bp，其开放阅读框（ORF）为2196 bp，编码一个732个氨基酸的蛋白质，包含五个HSPC家族特征序列，在其极端C末端具有MEEVD基序。HfHspc4全长2800 bp，其ORF为2364 bp，编码一个789个氨基酸的蛋白质，包含一个信号肽和五个家族特征序列，在其极端C末端具有HEEL基序。HfHspc1和HfHspc4的表达在雄性中最高，而在饱血雌性的卵巢中显著最高，这可能暗示了这些蛋白质在成功消化血液和卵子发育中的作用。
