Gu Yunlan, Qin Wenlong, Xu Haiyan, Liu Ying-Guo
School of Chemical and Environmental Engineering, Yancheng Teachers University, Yancheng, Jiangsu 224002, China.
School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212003, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Mar 15;329:125583. doi: 10.1016/j.saa.2024.125583. Epub 2024 Dec 12.
To address the toxicity concerns of aluminum ions (Al) due to their widespread environmental presence, a novel chromone-derived fluorescent probe, (E)-N'-((4-oxo-4H-chromen-3-yl)methylene)benzohydrazide (NMA), was developed for dual-mode detection combining colorimetric and fluorometric channels. Upon chelation with Al in a 1:1 stoichiometric ratio, NMA exhibited a significant fluorescence enhancement at 510 nm, accompanied by a rapid and visible color change due to the chelation-enhanced fluorescence (CHEF) effect, achieving an exceptional detection limit of 9 nM-well below the World Health Organization's recommended threshold. The reversible binding of NMA, demonstrated through sequential addition of Al and EDTA, enabled the construction of an INHIBIT molecular logic gate, broadening its potential for smart sensing applications. Structural interactions and selectivity were confirmed through H NMR, FT-IR spectroscopy, and density functional theory (DFT) calculations. NMA proved highly effective in diverse applications, including detecting Al in food samples, bioimaging in living cells, and environmental monitoring via smartphone-assisted platforms and test strips, making it a powerful tool for addressing aluminum toxicity in practical and real-time settings.
鉴于铝离子（Al）在环境中广泛存在而引发的毒性问题，开发了一种新型的基于色酮的荧光探针（E）-N'-（（4-氧代-4H-色烯-3-基）亚甲基）苯甲酰肼（NMA），用于结合比色和荧光通道的双模式检测。与Al以1:1化学计量比螯合时，NMA在510 nm处表现出显著的荧光增强，同时由于螯合增强荧光（CHEF）效应而伴随快速且明显的颜色变化，实现了9 nM的出色检测限——远低于世界卫生组织推荐的阈值。通过依次添加Al和EDTA证明了NMA的可逆结合，这使得能够构建一个INHIBIT分子逻辑门，拓宽了其在智能传感应用中的潜力。通过1H NMR、FT-IR光谱和密度泛函理论（DFT）计算证实了结构相互作用和选择性。NMA在多种应用中被证明非常有效，包括检测食品样品中的Al、活细胞中的生物成像以及通过智能手机辅助平台和测试条进行环境监测，使其成为在实际和实时环境中解决铝毒性问题的有力工具。
