Li Hongxia, Lin Qiqi, Zou Ruiqi, Zhang Meng, Liu Xin, Ye Haiqing, Sun Chunyan, Yan Xu
Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China.
Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China.
Biosens Bioelectron. 2025 Mar 1;271:117064. doi: 10.1016/j.bios.2024.117064. Epub 2024 Dec 13.
The construction of a nanozyme-enzyme hybrid cascade system is an effective protocol to optimize the performance of biosensors. Yet, the integration has limitations due to the lack of harmonious collaboration between nanozyme and enzyme. Herein, we have constructed an efficient enzymatic cascade system by utilizing the base complementary pairing and the targeting capability of DNA tweezers to combine DNA-regulated copper nanoflowers (CuNFs) with acetylcholinesterase (AChE). The DNA tweezers were immobilized onto the CuNFs undergo regular base complementary pairing, and subsequently employed as aptamer to capture AChE gently, forming CuNFs-Apt-AChE cascade system. This system not only enhanced the spatial proximity of CuNFs and AChE to increase cascade catalytic activity, but also demonstrated excellent stability under harsh conditions. Harnessing the nanoarchitecture and characteristics, the CuNFs-Apt-AChE composites were embedded into the hydrogel to fabricate a sensitive biosensor for on-site detecting carbamate pesticides with a detection limit of 0.19 ng mL. The hydrogel sensor exhibited high specificity for carbamate pesticides and had been successfully applied in water and juice samples for pesticide detection with strong anti-interference ability. This method holds great potential for the on-site detection of pesticides, offering a new strategy for constructing nanozyme-enzyme cascade hybrid systems with accuracy and sensitivity.
构建纳米酶-酶杂化级联系统是优化生物传感器性能的有效方案。然而，由于纳米酶与酶之间缺乏协同作用，这种整合存在局限性。在此，我们利用DNA镊子的碱基互补配对和靶向能力，将DNA调控的铜纳米花（CuNFs）与乙酰胆碱酯酶（AChE）相结合，构建了一种高效的酶促级联系统。DNA镊子通过常规碱基互补配对固定在CuNFs上，随后用作适配体来温和地捕获AChE，形成CuNFs-Apt-AChE级联系统。该系统不仅增强了CuNFs与AChE的空间接近性以提高级联催化活性，而且在恶劣条件下表现出优异的稳定性。利用这种纳米结构和特性，将CuNFs-Apt-AChE复合材料嵌入水凝胶中，制备了一种灵敏的生物传感器，用于现场检测氨基甲酸酯类农药，检测限为0.19 ng/mL。水凝胶传感器对氨基甲酸酯类农药表现出高特异性，并已成功应用于水和果汁样品中的农药检测，具有很强的抗干扰能力。该方法在农药现场检测方面具有巨大潜力，为构建具有准确性和灵敏度的纳米酶-酶级联杂化系统提供了一种新策略。
