Karimi Kudratullah, Zöller Jonathan, Hofmann Tommy, Zangl Rene, Schulte Jonathan, Langer Julian D, Schmidt Carla, Morgner Nina
Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, 60438 Frankfurt am Main, Germany.
Proteomics, Max-Planck-Institute for Biophysics, 60438 Frankfurt am Main, Germany.
J Am Soc Mass Spectrom. 2025 Jan 1;36(1):91-99. doi: 10.1021/jasms.4c00343. Epub 2024 Dec 17.
Electrospray mass spectrometry has become indispensable in many disciplines including the classic "omics" techniques such as proteomics or lipidomics, as well as other life science applications in molecular, cellular, and structural biology. However, a limiting factor that often arises for the detection of biomolecular analytes is their poor ionization efficiency in the ion source. Here, we present an add-on device for the electrospray source, termed MS (MS Spectral Impurity Eliminator & Value Enhancer), which is placed between the electrospray needle and the cone of the mass spectrometer. We probed the application of MS for various biomolecules including proteins, peptides, lipids, glycans and DNA oligonucleotides and even synthetic polymers such as polyethylene glycol and found that MS selectively improves the signal intensity, while suppressing the spectral contribution of contaminants such as NaCl. Importantly, MS can, in principle, be adapted for any electrospray ion source and, therefore, represents a promising alternative for routine "omics" methods as well as special applications on challenging analytes.
电喷雾质谱在许多学科中已变得不可或缺，这些学科包括蛋白质组学或脂质组学等经典的“组学”技术，以及分子生物学、细胞生物学和结构生物学中的其他生命科学应用。然而，生物分子分析物检测中经常出现的一个限制因素是它们在离子源中的电离效率较低。在此，我们展示了一种用于电喷雾源的附加装置，称为MS （质谱谱图杂质消除器和值增强器），它放置在电喷雾针和质谱仪的锥孔之间。我们探究了MS 在包括蛋白质、肽、脂质、聚糖和DNA寡核苷酸等各种生物分子甚至聚乙二醇等合成聚合物上的应用，发现MS 能选择性地提高信号强度，同时抑制诸如氯化钠等污染物的谱图贡献。重要的是，MS 在原则上可适用于任何电喷雾离子源，因此，对于常规的“组学”方法以及对具有挑战性的分析物的特殊应用而言，它是一种很有前景的替代方法。
